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BMC Pulmonary Medicine

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Open Access 01-12-2020 | Lung Cancer | Research article

miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis

Authors: Jin-shan Zhou, Ze-shan Yang, Si-yang Cheng, Jiang-hao Yu, Chao-Jun Huang, Qiang Feng

Published in: BMC Pulmonary Medicine | Issue 1/2020

Abstract

Background

miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis.

Methods

Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. Luciferase reporter assays were used to illustrate the interaction between miR-425-5p and PTEN.

Results

We demonstrate that miR-425-5p is overexpressed in LCa tissue and enhances the proliferative and colony formation capacity of the LCa cell lines A549 and NCI-H1299. Through predictive binding assays, PTEN was identified as a direct gene target and its exogenous expression inhibited the pro-cancer effects of miR-425-5p. Through its ability to down-regulate PTEN, miR-425-5p activated the PI3K/AKT axis.

Conclusion

We conclude that miR-425-5p promotes LCa tumorigenesis through PTEN/PI3K/AKT signaling.

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Title: miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis
Authors: Jin-shan Zhou
Ze-shan Yang
Si-yang Cheng
Jiang-hao Yu
Chao-Jun Huang
Qiang Feng
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Lung Cancer
Lung Cancer
Lung Cancer
Published in: BMC Pulmonary Medicine / Issue 1/2020
Electronic ISSN: 1471-2466
DOI: https://doi.org/10.1186/s12890-020-01261-0

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miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis

Abstract

Background

Methods

Results

Conclusion

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Abstract

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