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BMC Cancer
Published in: BMC Cancer 1/2018

Open Access 01-12-2018 | Research article

Frequency of HPV in oral cavity squamous cell carcinoma

Authors: Priscila Marinho de Abreu, Anna Clara Gregório Có, Pedro Leite Azevedo, Isabella Bittencourt do Valle, Karine Gadioli de Oliveira, Sônia Alves Gouvea, Melissa Freitas Cordeiro-Silva, Iúri Drummond Louro, José Roberto Vasconcelos de Podestá, Jeferson Lenzi, Agenor Sena, Elismauro Francisco Mendonça, Sandra Lúcia Ventorin von Zeidler

Published in: BMC Cancer | Issue 1/2018

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Abstract

Background

The prevalence of high-risk human papillomavirus (HPV) DNA in cases of oral cavity squamous cell carcinoma (SCC) varies widely. The aim of this study is to investigate the frequency of high-risk HPV DNA in a large Brazilian cohort of patients with oral cavity SCC.

Methods

Biopsy and resected frozen and formalin-fixed paraffin-embedded specimens of oral cavity SCC were available from 101 patients who were recruited at two Brazilian centres. Stringent measures with respect to case selection and prevention of sample contamination were adopted to ensure reliability of the data. Nested PCR using MY09/MY11 and GP5+/GP6+ as well as PGMY09/11 L1 consensus primers were performed to investigate the presence of HPV DNA in the tumours. HPV-positive cases were subjected to direct sequencing. Shapiro–Wilk and Student t test were used to evaluate data normality and to compare the means, respectively. Qualitative variables were analysed by logistic regression.

Results

Our results demonstrate that the frequency of high-risk HPV types in oral cavity SCC is very low and is less than 4%. All HPV-positive cases were HPV16. In addition, our results do not show a significant association between the tumour clinical features and the risk factors (tobacco, alcohol and HPV) for oral cavity SCC.

Conclusion

In the current study, we observed an overlapping pattern of risk factors that are related to tumour development. This, along with a low frequency of high-risk HPV DNA, supports the findings that HPV is not involved in the genesis of oral cavity SCC in Brazilian population.
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Metadata
Title
Frequency of HPV in oral cavity squamous cell carcinoma
Authors
Priscila Marinho de Abreu
Anna Clara Gregório Có
Pedro Leite Azevedo
Isabella Bittencourt do Valle
Karine Gadioli de Oliveira
Sônia Alves Gouvea
Melissa Freitas Cordeiro-Silva
Iúri Drummond Louro
José Roberto Vasconcelos de Podestá
Jeferson Lenzi
Agenor Sena
Elismauro Francisco Mendonça
Sandra Lúcia Ventorin von Zeidler
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-018-4247-3

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