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Open Access 01-12-2021 | Human Papillomavirus | Research article

Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

Authors: Akouélé P. Kuassi-Kpede, Essolakina Dolou, Théodora M. Zohoncon, Ina Marie Angèle Traore, Gnatoulma Katawa, R. Alice Ouedraogo, Esther Mah Traore, Prosper Bado, T. Clarisse Ouedraogo, Florencia Wendkuuni Djigma, Simplice Damintoti Karou, Jacques Simpore

Published in: BMC Infectious Diseases | Issue 1/2021

Abstract

Background

The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women.

Methods

Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR.

Results

Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL.

Conclusion

This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

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Title: Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo
Authors: Akouélé P. Kuassi-Kpede
Essolakina Dolou
Théodora M. Zohoncon
Ina Marie Angèle Traore
Gnatoulma Katawa
R. Alice Ouedraogo
Esther Mah Traore
Prosper Bado
T. Clarisse Ouedraogo
Florencia Wendkuuni Djigma
Simplice Damintoti Karou
Jacques Simpore
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Human Papillomavirus
Cervical Cancer
Cervical Cancer
Polymerase Chain Reaction
Published in: BMC Infectious Diseases / Issue 1/2021
Electronic ISSN: 1471-2334
DOI: https://doi.org/10.1186/s12879-021-05956-5

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Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

Abstract

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Methods

Results

Conclusion

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Abstract

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Conclusion

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