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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2020

01-12-2020 | Expectoration | Research article

Altered respiratory microbiota composition and functionality associated with asthma early in life

Authors: Mohammad T. AL Bataineh, Rifat A. Hamoudi, Nihar R. Dash, Rakhee K. Ramakrishnan, Mohamad A. Almasalmeh, Hanan A. Sharif, Mohamed S. Al-Hajjaj, Qutayba Hamid

Published in: BMC Infectious Diseases | Issue 1/2020

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Abstract

Background

The microbiota of the respiratory tract has an important role in maintaining respiratory health. However, little is known on the respiratory microbiota in asthmatic patients among Middle Eastern populations. This study investigated the respiratory microbiota composition and functionality associated with asthma in Emirati subjects.

Methods

We performed 16S rRNA and ITS2-gene based microbial profiling of 40 expectorated sputum samples from adult and pediatric Emirati individuals averaging 52 and 7 years of age, respectively with or without asthma.

Results

We report bacterial difference belonging to Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria phyla between asthmatic and non-asthmatic controls. Similarly, fungal difference belonging to Ascomycota, Basidiomycota phyla and other unclassified fungi. Differential abundance testing among asthmatic individuals with relation to Asthma Control Test show a significant depletion of Penicillium aethiopicum and Alternaria spp., among poorly controlled asthmatics. Moreover, data suggest a significant expansion of Malassezia spp. and other unclassified fungi in the airways of those receiving steroids and leukotriene receptor antagonists’ combination therapy, in contrast to those receiving steroids alone. Functional profiling from 16S data showed marked differences between pediatric asthmatic and non-asthmatic controls, with pediatric asthmatic patients showing an increase in amino acid (p-value < 5.03 × 10− 7), carbohydrate (p-value < 4.76 × 10− 7), and fatty acid degradation (p-value < 6.65 × 10− 7) pathways, whereas non-asthmatic controls are associated with increase in amino acid (p-value < 8.34 × 10− 7), carbohydrate (p-value < 3.65 × 10− 7), and fatty acid (p-value < 2.18 × 10− 6) biosynthesis pathways in concordance with enterotype composition.

Conclusions

These differences provide an insight into respiratory microbiota composition in Emirati population and its possible role in the development of asthma early in life. This study provides important information that may eventually lead to the development of screening biomarkers to predict early asthma development and novel therapeutic approaches.
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Metadata
Title
Altered respiratory microbiota composition and functionality associated with asthma early in life
Authors
Mohammad T. AL Bataineh
Rifat A. Hamoudi
Nihar R. Dash
Rakhee K. Ramakrishnan
Mohamad A. Almasalmeh
Hanan A. Sharif
Mohamed S. Al-Hajjaj
Qutayba Hamid
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2020
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/s12879-020-05427-3

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