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BMC Cardiovascular Disorders
Published in: BMC Cardiovascular Disorders 1/2021

01-12-2021 | Tetralogy of Fallot | Research article

Construction and investigation of a circRNA-associated ceRNA regulatory network in Tetralogy of Fallot

Authors: Haifei Yu, Xinrui Wang, Hua Cao

Published in: BMC Cardiovascular Disorders | Issue 1/2021

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Abstract

Background

As the most frequent type of cyanotic congenital heart disease (CHD), tetralogy of Fallot (TOF) has a relatively poor prognosis without corrective surgery. Circular RNAs (circRNAs) represent a novel class of endogenous noncoding RNAs that regulate target gene expression posttranscriptionally in heart development. Here, we investigated the potential role of the ceRNA network in the pathogenesis of TOF.

Methods

To identify circRNA expression profiles in TOF, microarrays were used to screen the differentially expressed circRNAs between 3 TOF and 3 control human myocardial tissue samples. Then, a dysregulated circRNA-associated ceRNA network was constructed using the established multistep screening strategy.

Results

In summary, a total of 276 differentially expressed circRNAs were identified, including 214 upregulated and 62 downregulated circRNAs in TOF samples. By constructing the circRNA-associated ceRNA network based on bioinformatics data, a total of 19 circRNAs, 9 miRNAs, and 34 mRNAs were further screened. Moreover, by enlarging the sample size, the qPCR results validated the positive correlations between hsa_circ_0007798 and HIF1A.

Conclusions

The findings in this study provide a comprehensive understanding of the ceRNA network involved in TOF biology, such as the hsa_circ_0007798/miR-199b-5p/HIF1A signalling axis, and may offer candidate diagnostic biomarkers or potential therapeutic targets for TOF. In addition, we propose that the ceRNA network regulates TOF progression.
Appendix
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Metadata
Title
Construction and investigation of a circRNA-associated ceRNA regulatory network in Tetralogy of Fallot
Authors
Haifei Yu
Xinrui Wang
Hua Cao
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Cardiovascular Disorders / Issue 1/2021
Electronic ISSN: 1471-2261
DOI
https://doi.org/10.1186/s12872-021-02217-w

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