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Open Access 01-12-2017 | Research article

Endothelial progenitor cells and plaque burden in stented coronary artery segments: an optical coherence tomography study six months after elective PCI

Authors: Sylvia Otto, Kristina Nitsche, Christian Jung, Aleh Kryvanos, Andrey Zhylka, Kerstin Heitkamp, Juan-Luis Gutiérrez-Chico, Björn Goebel, P. Christian Schulze, Hans R. Figulla, Tudor C. Poerner

Published in: BMC Cardiovascular Disorders | Issue 1/2017

Abstract

Background

Endothelial progenitor cells (EPC) are involved in neovascularization and endothelial integrity. They might be protective in atherosclerosis. Optical coherence tomography (OCT) is a precise intracoronary imaging modality that allows assessment of subintimal plaque development. We evaluated the influence of EPC on coronary plaque burden in stable disease and implemented a novel computational plaque analysis algorithm using OCT.

Methods

Forty-three patients (69.8% males, 69.6 ± 7.7 years) were investigated by OCT during re-angiography 6 months after elective stent implantation. Different subpopulations of EPCs were identified by flow cytometry according to their co-expression of antigens (CD34+, CD133+, kinase domain receptor, KDR+). An algorithm was applied to calculate the underlying total plaque burden of the stented segments from OCT images. Plaque morphology was assessed according to international consensus in OCT imaging.

Results

A cumulative sub-strut plaque volume of 10.87 ± 12.7 mm³ and a sub-stent plaque area of 16.23 ± 17.0 mm² were found within the stented vessel segments with no significant differences between different stent types. All EPC subpopulations (mean of EPC levels: CD34+/CD133+: 2.66 ± 2.0%; CD34+/KDR+: 7.50 ± 5.0%; CD34+/CD133+/KDR+: 1.12 ± 1.0%) inversely correlated with the identified underlying total plaque volume and plaque area (p ≤ 0.012).

Conclusions

This novel analysis algorithm allows for the first time comprehensive quantification of coronary plaque burden by OCT and illustration as spread out vessel charts. Increased EPC levels are associated with less sub-stent coronary plaque burden which adds to previous findings of their protective role in atherosclerosis.

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Title: Endothelial progenitor cells and plaque burden in stented coronary artery segments: an optical coherence tomography study six months after elective PCI
Authors: Sylvia Otto
Kristina Nitsche
Christian Jung
Aleh Kryvanos
Andrey Zhylka
Kerstin Heitkamp
Juan-Luis Gutiérrez-Chico
Björn Goebel
P. Christian Schulze
Hans R. Figulla
Tudor C. Poerner
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Cardiovascular Disorders / Issue 1/2017
Electronic ISSN: 1471-2261
DOI: https://doi.org/10.1186/s12872-017-0534-1

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Abstract

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Results

Conclusions

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