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Open Access 01-12-2018 | Research article

The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis

Authors: Milad Sabzevary-Ghahfarokhi, Mojtaba Shohan, Hedayatollah Shirzad, Ghorbanali Rahimian, Nader Bagheri, Amin Soltani, Fatemeh Deris, Mahdi Ghatreh-Samani, Ehsan Razmara

Published in: BMC Immunology | Issue 1/2018

Abstract

Background

Fra-1 (fosl1) belongs to the activator protein1 (AP-1) family inducing IL-11 expression in oxidative stress condition. IL-11 plays a pivotal role in protecting epithelial barriers integrity. In this study, we investigated the Fra-1 gene expression in the inflamed mucosa of patients with ulcerative colitis (UC) as well as its relation to IL-11 expression.

Materials and methods

We enrolled 20 patients and 20 healthy controls with definite UC based on the clinical criteria. Fra-1 gene expression in inflamed and non-inflamed colonic biopsies was determined by real-time polymerase chain reaction (RT-PCR). The IL-11 protein concentration was measured by Enzyme-Linked Immunosorbent Assay (ELISA) method. Pearson correlation was applied to calculate the relation between Fra-1 and IL-11.

Results

An increased level of Fra-1 gene expression was observed in patients with mild ulcerative colitis. The protein concentration of IL-11 was also increased in mild UC patients. Conversely, a significant decrease of IL-11 protein level was detected in severe UC patients compared to control group.

Conclusion

Oxidative stress in inflamed intestinal biopsies can induce fra-1 gene expression. Our findings suggest that Fra-1 transcription factor leads to the production of IL-11 protein in UC patients.

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Title: The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis
Authors: Milad Sabzevary-Ghahfarokhi
Mojtaba Shohan
Hedayatollah Shirzad
Ghorbanali Rahimian
Nader Bagheri
Amin Soltani
Fatemeh Deris
Mahdi Ghatreh-Samani
Ehsan Razmara
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Immunology / Issue 1/2018
Electronic ISSN: 1471-2172
DOI: https://doi.org/10.1186/s12865-018-0257-9

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The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis

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Background

Materials and methods

Results

Conclusion

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