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BMC Immunology
Published in: BMC Immunology 1/2018

Open Access 01-12-2018 | Research Article

RNA gene profile variation in peripheral blood mononuclear cells from rhesus macaques immunized with Hib conjugate vaccine, Hib capsular polysaccharide and TT carrier protein

Authors: Jing Tang, Ying Zhang, Xiaolong Zhang, Yun Liao, Yongrong Wang, Shengjie Ouyang, Yanchun Che, Miao Xu, Jing Pu, Qi Shen, Zhanlong He, Qiang Ye, Qihan Li

Published in: BMC Immunology | Issue 1/2018

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Abstract

Background

The Haemophilus influenzae type b (Hib) conjugate vaccine has been widely used in children to prevent invasive Hib disease because of its strong immunogenicity and antibody response induction relative to the capsular polysaccharide (CPS) antigen. The data from vaccine studies suggest that the conjugate vaccine contains carrier proteins that enhance and/or regulate the antigen’s immunogenicity, but the mechanism of this enhancement remains unclear.

Methods

To explore the immunological role of the conjugate vaccine, we compared the immune responses and gene profiles of rhesus macaques after immunization with CPS, carrier protein tetanus toxoid (TT) or conjugate vaccine.

Results

A distinct immune response was induced by the Hib conjugate vaccine but not by CPS or carrier protein TT. The genes that were dynamically regulated in conjunction with the macaque immune responses to the conjugate vaccine were investigated.

Conclusions

We propose that these genes are involved in the induction of specific immunity that is characterized by the appearance and maintenance of antibodies against Hib.
Appendix
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Metadata
Title
RNA gene profile variation in peripheral blood mononuclear cells from rhesus macaques immunized with Hib conjugate vaccine, Hib capsular polysaccharide and TT carrier protein
Authors
Jing Tang
Ying Zhang
Xiaolong Zhang
Yun Liao
Yongrong Wang
Shengjie Ouyang
Yanchun Che
Miao Xu
Jing Pu
Qi Shen
Zhanlong He
Qiang Ye
Qihan Li
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2018
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/s12865-018-0240-5

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