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BMC Immunology
Published in: BMC Immunology 1/2017

Open Access 01-12-2017 | Research article

Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling

Authors: Xi-Yao Huang, Abdur Rahman Ansari, Hai-Bo Huang, Xing Zhao, Ning-Ya Li, Zhi-Jian Sun, Ke-Mei Peng, Juming Zhong, Hua-Zhen Liu

Published in: BMC Immunology | Issue 1/2017

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Abstract

Background

Lipopolysaccharide (LPS) induces acute liver injury and the complex mechanisms include the activation of toll like receptor 4 (TLR4) signaling pathway in many species. However, immuno-pathological changes during TLR4 signaling under LPS stress in acute liver injury is poorly understood in avian species. The present investigation was therefore carried out to evaluate these alterations in TLR4 signaling pathway during acute liver injury in young chickens.

Results

After intraperitoneal injection of LPS or saline, liver samples were harvested at 0, 2, 6, 12, 24, 36, 72 and 120 h (n = 6 at each time point) and the microstructures were analyzed by hematoxylin and eosin (H&E) staining. Alanine aminotransferase (ALT) and caspase-3 enzyme activity was assessed by enzyme-linked immunosorbent assay (ELISA). Proliferative cell nuclear antigen (PCNA), single stranded DNA (ssDNA) and TLR4 protein expressions were determined by immunohistochemistry. Gene expressions of PCNA, caspase-3, caspase-8, TLR4 and its downstream molecules were analyzed by quantitative polymerase chain reaction (qPCR). LPS injection induced significantly higher ALT activity, severe fatty degeneration, necrotic symptoms, ballooning degeneration, congestion, enhanced inflammatory cell infiltration in liver sinusoids, decreased proliferation, increased apoptosis and significant up-regulation in TLR4 and its downstream molecules (MyD88, NF-κB, TNF-α, IL-1β and TGF-β) expression at different time points.

Conclusions

This study indicated that TLR4 signaling and its downstream molecules along with certain cytokines play a key role in acute liver injury in young chickens. Hence, our findings provided novel information about the histopathological, proliferative and apoptotic alterations along with changes in ALT and caspase-3 activities associated with acute liver injury induced by Salmonella LPS in avian species.
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Metadata
Title
Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling
Authors
Xi-Yao Huang
Abdur Rahman Ansari
Hai-Bo Huang
Xing Zhao
Ning-Ya Li
Zhi-Jian Sun
Ke-Mei Peng
Juming Zhong
Hua-Zhen Liu
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2017
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/s12865-017-0199-7

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