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Open Access 01-12-2014 | Research article

Anti-cancer properties of gastropodan hemocyanins in murine model of colon carcinoma

Authors: Vera Gesheva, Stela Chausheva, Nikolina Mihaylova, Iliyan Manoylov, Lyuba Doumanova, Krassimira Idakieva, Andrey Tchorbanov

Published in: BMC Immunology | Issue 1/2014

Abstract

Background

Various immunotherapeutic approaches have been used for the treatment of cancer. A number of natural compounds are designed to repair, stimulate, or enhance the immune system response. Among them are the hemocyanins (Hcs) - extracellular copper proteins isolated from different arthropod and mollusc species. Hcs are oxygen transporter molecules and normally are freely dissolved in the hemolymph of these animals. Hemocyanins are very promising class of anti-cancer therapeutics due to their immunogenic properties and the absence of toxicity or side effects. KLH (Megathura crenulata hemocyanin) is the most studied molecule of this group setting a standard for natural carrier protein for small molecules and has been used in anti-tumor clinical trials.

Results

The Hcs isolated from marine snail Rapana thomasiana (RtH) and the terrestrial snail Helix pomatia (HpH) express strong in vivo anti-cancer and anti-proliferative effects in the developed by us murine model of colon carcinoma. The immunization with RtH and HpH prolonged the survival of treated animals, improve humoral anti-cancer response and moderate the manifestation of C-26 carcinoma symptoms as tumor growth, splenomegaly and lung metastasis appearance.

Conclusion

Hemocyanins are used so far for therapy of superficial bladder cancer and murine melanoma models. Our findings demonstrate a potential anti-cancer effect of hemocyanins on a murine model of colon carcinoma suggesting their use for immunotherapy of different types of cancer.

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Title: Anti-cancer properties of gastropodan hemocyanins in murine model of colon carcinoma
Authors: Vera Gesheva
Stela Chausheva
Nikolina Mihaylova
Iliyan Manoylov
Lyuba Doumanova
Krassimira Idakieva
Andrey Tchorbanov
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Immunology / Issue 1/2014
Electronic ISSN: 1471-2172
DOI: https://doi.org/10.1186/s12865-014-0034-3

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Anti-cancer properties of gastropodan hemocyanins in murine model of colon carcinoma

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