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Open Access 01-10-2010 | Research

Open lung approach associated with high-frequency oscillatory or low tidal volume mechanical ventilation improves respiratory function and minimizes lung injury in healthy and injured rats

Authors: Joerg Krebs, Paolo Pelosi, Charalambos Tsagogiorgas, Liesa Zoeller, Patricia RM Rocco, Benito Yard, Thomas Luecke

Published in: Critical Care | Issue 5/2010

Abstract

Introduction

To test the hypothesis that open lung (OL) ventilatory strategies using high-frequency oscillatory ventilation (HFOV) or controlled mechanical ventilation (CMV) compared to CMV with lower positive end-expiratory pressure (PEEP) improve respiratory function while minimizing lung injury as well as systemic inflammation, a prospective randomized study was performed at a university animal laboratory using three different lung conditions.

Methods

Seventy-eight adult male Wistar rats were randomly assigned to three groups: (1) uninjured (UI), (2) saline washout (SW), and (3) intraperitoneal/intravenous Escherichia coli lipopolysaccharide (LPS)-induced lung injury. Within each group, animals were further randomized to (1) OL with HFOV, (2) OL with CMV with "best" PEEP set according to the minimal static elastance of the respiratory system (BP-CMV), and (3) CMV with low PEEP (LP-CMV). They were then ventilated for 6 hours. HFOV was set with mean airway pressure (P_meanHFOV) at 2 cm H₂O above the mean airway pressure recorded at BP-CMV (P_meanBP-CMV) following a recruitment manoeuvre. Six animals served as unventilated controls (C). Gas-exchange, respiratory system mechanics, lung histology, plasma cytokines, as well as cytokines and types I and III procollagen (PCI and PCIII) mRNA expression in lung tissue were measured.

Results

We found that (1) in both SW and LPS, HFOV and BP-CMV improved gas exchange and mechanics with lower lung injury compared to LP-CMV, (2) in SW; HFOV yielded better oxygenation than BP-CMV; (3) in SW, interleukin (IL)-6 mRNA expression was lower during BP-CMV and HFOV compared to LP-CMV, while in LPS inflammatory response was independent of the ventilatory mode; and (4) PCIII mRNA expression decreased in all groups and ventilatory modes, with the decrease being highest in LPS.

Conclusions

Open lung ventilatory strategies associated with HFOV or BP-CMV improved respiratory function and minimized lung injury compared to LP-CMV. Therefore, HFOV with Pmean_HFOV set 2 cm H₂O above the Pmean_BP-CMV following a recruitment manoeuvre is as beneficial as BP-CMV.

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Title: Open lung approach associated with high-frequency oscillatory or low tidal volume mechanical ventilation improves respiratory function and minimizes lung injury in healthy and injured rats
Authors: Joerg Krebs
Paolo Pelosi
Charalambos Tsagogiorgas
Liesa Zoeller
Patricia RM Rocco
Benito Yard
Thomas Luecke
Publication date: 01-10-2010
Publisher: BioMed Central
Published in: Critical Care / Issue 5/2010
Electronic ISSN: 1364-8535
DOI: https://doi.org/10.1186/cc9291

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Open lung approach associated with high-frequency oscillatory or low tidal volume mechanical ventilation improves respiratory function and minimizes lung injury in healthy and injured rats

Abstract

Introduction

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusions

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