Published in:
01-05-2010 | Poster presentation
Modelling breast cancer in a three-dimensional heterotypic culture system
Authors:
DL Holliday, S Maltby, MA Moss, AM Hanby, JL Jones, V Speirs
Published in:
Breast Cancer Research
|
Special Issue 1/2010
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Excerpt
Microenvironmental factors are fundamental in the regulation of normal and tumour breast tissue. Two cell types have been implicated in having opposing effects on breast tumour cell behaviour: myoepithelial cells exhibit broad tumour-suppressor activity, whilst fibroblasts frequently promote tumour growth and invasion. Previous work has described the development of a physiologically relevant three-dimensional heterotypic culture system containing tumour, myoepithelial and fibroblast cells. The data showed organisation of the cells into co-unit structures recapitulating ductal carcinoma in situ breast, with homing of myoepithelial cells around luminal cells, and highlighted a central role for tumour-associated fibroblasts in disrupting ductal carcinoma in situ structures. This study describes further manipulation of the model to include tumour cells that represent the heterogeneity of breast cancer. …