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Breast Cancer Research
Published in: Breast Cancer Research 1/2010

01-05-2010 | Poster presentation

Evaluating gene expression in formalin-fixed, paraffin-embedded breast cancer tissues using DASL®

Authors: T Burr, R Dixon, A Green, I Ellis, C Murray

Published in: Breast Cancer Research | Special Issue 1/2010

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Excerpt

The study of gene expression in conventionally processed tissues is hampered by degradation of mRNA. Expensive, low-multiplex, quantitative PCR methods can be unreliable due to the limited template sizes. One way to overcome this problem is to use array-based methods. DASL® technology relies on random priming for production of cDNA, in concert with universal bead arrays to allow the detection and relative quantitation of expression of specific gene subsets. …
Metadata
Title
Evaluating gene expression in formalin-fixed, paraffin-embedded breast cancer tissues using DASL®
Authors
T Burr
R Dixon
A Green
I Ellis
C Murray
Publication date
01-05-2010
Publisher
BioMed Central
Published in
Breast Cancer Research / Issue Special Issue 1/2010
Electronic ISSN: 1465-542X
DOI
https://doi.org/10.1186/bcr2531

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