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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 1/2009

Open Access 01-02-2009 | Research article

The proliferative human monocyte subpopulation contains osteoclast precursors

Authors: Roya Lari, Peter D Kitchener, John A Hamilton

Published in: Arthritis Research & Therapy | Issue 1/2009

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Abstract

Introduction

Immediate precursors of bone-resorbing osteoclasts are cells of the monocyte/macrophage lineage. Particularly during clinical conditions showing bone loss, it would appear that osteoclast precursors are mobilized from bone marrow into the circulation prior to entering tissues undergoing such loss. The observed heterogeneity of peripheral blood monocytes has led to the notion that different monocyte subpopulations may have special or restricted functions, including as osteoclast precursors.

Methods

Human peripheral blood monocytes were sorted based upon their degree of proliferation and cultured in macrophage colony-stimulating factor (M-CSF or CSF-1) and receptor activator of nuclear factor-kappa-B ligand (RANKL).

Results

The monocyte subpopulation that is capable of proliferation gave rise to significantly more multinucleated, bone-resorbing osteoclasts than the bulk of the monocytes.

Conclusions

Human peripheral blood osteoclast precursors reside in the proliferative monocyte subpopulation.
Appendix
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Metadata
Title
The proliferative human monocyte subpopulation contains osteoclast precursors
Authors
Roya Lari
Peter D Kitchener
John A Hamilton
Publication date
01-02-2009
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 1/2009
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/ar2616

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