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Gut Pathogens
Published in: Gut Pathogens 1/2014

Open Access 01-12-2014 | Research

A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles

Authors: Zhiqiang Shen, Nannan Hou, Min Jin, Zhigang Qiu, Jingfeng Wang, Bin Zhang, Xinwei Wang, Jie Wang, Dongsheng Zhou, Junwen Li

Published in: Gut Pathogens | Issue 1/2014

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Abstract

This paper presents a functional nanoparticle-enhanced enzyme-linked immunosorbent assay (FNP-ELISA) for detection of enterohemorrhagic Escherichia coli (EHEC) O157:H7. Immunomagnetic nanoparticles (IMMPs) conjugated with monoclonal anti-O157:H7 antibody were used to capture E. coli O157:H7. Beacon gold nanoparticles (B-GNPs) coated with polyclonal anti-O157:H7 and biotin single-stranded DNA (B-DNA) were then subjective to immunoreaction with E. coli O157:H7, which was followed by streptavidin-horseradish peroxidase (Strep-HRP) conjugated with B-GNPs based on a biotin-avidin system. The solutions containing E. coli O157:H7, IMMPs, B-GNPs, and Strep-HRP were collected for detecting color change. The signal was significantly amplified with detection limits of 68 CFU mL-1 in PBS and 6.8 × 102 to 6.8 × 103 CFU mL-1 in the food samples. The FNP-ELISA method developed in this study was two orders of magnitude more sensitive than immunomagnetic separation ELISA (IMS-ELISA) and four orders of magnitude more sensitive than C-ELISA. The entire detection process of E. coli O157:H7 lasted only 3 h, and thus FNP-ELISA is considered as a time-saving method.
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Metadata
Title
A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles
Authors
Zhiqiang Shen
Nannan Hou
Min Jin
Zhigang Qiu
Jingfeng Wang
Bin Zhang
Xinwei Wang
Jie Wang
Dongsheng Zhou
Junwen Li
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Gut Pathogens / Issue 1/2014
Electronic ISSN: 1757-4749
DOI
https://doi.org/10.1186/1757-4749-6-14

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