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Open Access 01-12-2013 | Research

Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses

Authors: Jiayi Zhu, Qing Yang, Liyan Cao, Xiujing Dou, Jianguo Zhao, Weijuan Zhu, Fan Ding, Ri-e Bu, Siqingaowa Suo, Yudong Ren, Guangxing Li, Xiaofeng Ren

Published in: Virology Journal | Issue 1/2013

Abstract

Background

The context and purpose of the study included 1) bacterial expression of viral protein 6 (VP6) of porcine rotavirus (PRV) and generation of rabbit polyclonal antiserum to the VP6 protein; 3) establishment of a discrimination ELISA to distinguish PRV from a panel of other porcine viruses.

Results

The VP6 gene of PRV isolate DN30209 amplified by reverse transcription-PCR was 1356 bp containing a complete open reading frame (ORF) encoding 397 amino acids. Sequence comparison and phylogenetic analysis indicated that PRV DN30209 may belong to group A of rotavirus. Bacterially expressed VP6 was expressed in E.coli and anti-VP6 antibody was capable of distinguishing PRV from Porcine transmissible gastroenteritis virus, Porcine epidemic diarrhea virus, Porcine circovirus type II, Porcine reproductive and respiratory syndrome virus, Porcine pseudorabies virus and Porcine parvovirus.

Conclusions

PRV VP6 expressed in E. coli can be used to generate antibodies in rabbit; anti-VP6 serum antibody can be used as good diagnostic reagents for detection of PRV.

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Title: Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses
Authors: Jiayi Zhu
Qing Yang
Liyan Cao
Xiujing Dou
Jianguo Zhao
Weijuan Zhu
Fan Ding
Ri-e Bu
Siqingaowa Suo
Yudong Ren
Guangxing Li
Xiaofeng Ren
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2013
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-10-91

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Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses

Abstract

Background

Results

Conclusions

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