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Journal of Ovarian Research

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Open Access 01-12-2013 | Research

Altered expression of miRNAs in a dihydrotestosterone-induced rat PCOS model

Authors: Md Munir Hossain, Mingju Cao, Qi Wang, Ji Young Kim, Karl Schellander, Dawit Tesfaye, Benjamin K Tsang

Published in: Journal of Ovarian Research | Issue 1/2013

Abstract

Background

The polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine condition characterized by hyperandrogenism, hyperinsulinemia, insulin resistance and chronic anovulation. Regulation and interaction of a multitude of genes required for follicular development are found to be altered in PCOS. MicroRNAs (miRNAs) mediate posttranscriptional gene regulation by binding to the 3´ untranslated region of mRNAs to either inhibit or enhance translation. However, the extent and regulation of miRNA expression in PCOS is poorly understood and the current study is the first to describe altered expression of miRNAs in PCOS.

Methods

A chronically androgenized [5α-dihydrotestosterone (DHT)-treated] rat model which recapitulates many of the phenotypes of human PCOS, and miRNA PCR array was used to investigate the expression of 349 miRNAs in DHT treated rat ovaries. The ovarian expression of several selected miRNAs was also analyzed by in situ localization experiment.

Results

DHT-treated rats exhibit increased body weight, disrupted estrus cyclicity, decreased insulin sensitivity and decreased ovarian weight, with the latter phenomenon readily rescued by gonadotropin treatment in vivo. In general, 24% of the 349 miRNAs investigated were found to be differentially expressed between DHT-treated and control rats. Most of the differentially expressed miRNAs were found to be predominantly localized in the theca cells of the follicles. In silico analysis of the potential target genes of dysregulated miRNAs revealed their possible involvement in various pathways in the regulation of ovarian function.

Conclusion

Our current findings suggest that miRNAs are differentially regulated in hyperandrogenism, a condition possibly involved in the dysregulation of steroid hormone receptors and intra-ovarian factors, and that miRNAs may be involved in the etiology of PCOS.

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Title: Altered expression of miRNAs in a dihydrotestosterone-induced rat PCOS model
Authors: Md Munir Hossain
Mingju Cao
Qi Wang
Ji Young Kim
Karl Schellander
Dawit Tesfaye
Benjamin K Tsang
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2013
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/1757-2215-6-36

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Altered expression of miRNAs in a dihydrotestosterone-induced rat PCOS model

Abstract

Background

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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