Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Endocrine
Published in: Endocrine 2/2010

01-10-2010 | Original Article

Follicle-stimulating hormone regulation of microRNA expression on progesterone production in cultured rat granulosa cells

Authors: Nan Yao, Bai-Qing Yang, Yu Liu, Xin-Yu Tan, Cai-Ling Lu, Xiao-Hua Yuan, Xu Ma

Published in: Endocrine | Issue 2/2010

Login to get access

Abstract

MicroRNAs (miRNAs) regulate gene expression post-transcriptionally by interacting with the 3′ untranslated regions of their target mRNAs. Previously, miRNAs have been shown to regulate genes involved in cell growth, apoptosis, and differentiation, but their role in ovarian granulosa cell follicle-stimulating hormone (FSH)-stimulated steroidogenesis is unclear. Here we show that expression of 31 miRNAs is altered during FSH-mediated progesterone secretion of cultured granulosa cells. Specifically, 12 h after FSH treatment, miRNAs mir-29a and mir-30d were significantly down-regulated. However, their expression increased after 48 h. Bioinformatic analysis used to predict potential targets of mir-29a and mir-30d revealed a wide array of potential mRNA target genes, including those encoding genes involved in multiple signaling pathways. Taken together, our results pointed to a novel mechanism for the pleiotropic effects of FSH.
Appendix
Available only for authorised users
Literature
1.
T.R. Kumar, Y. Wang, N. Lu, M.M. Matzuk, Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat. Genet. 15, 201–204 (1997)CrossRefPubMed
2.
A. Amsterdam, N. Selvaraj, Control of differentiation, transformation, and apoptosis in granulosa cells by oncogenes, oncoviruses, and tumor suppressor genes. Endocr. Rev. 18, 435–461 (1997)CrossRefPubMed
3.
A. Amsterdam, S. Rotmensch, A. Ben-Ze’ev, Coordinated regulation of morphological and biochemical differentiation in a steroidogenic cell: the granulosa cell model. Trends Biochem. Sci. 14, 377–382 (1989)CrossRefPubMed
4.
A. Amsterdam, R.S. Gold, K. Hosokawa, Y. Yoshida, R. Sasson, Y. Jung, F. Kotsuji, Crosstalk among multiple signaling pathways controlling ovarian cell death. Trends Endocrinol. Metab. 10, 255–262 (1999)CrossRefPubMed
5.
R.L. Robker, D.L. Russell, S. Yoshioka, S.C. Sharma, J.P. Lydon, B.W. O’Malley, L.L. Espey, J.S. Richards, Ovulation: a multi-gene, multi-step process. Steroids 65, 559–570 (2000)CrossRefPubMed
6.
N.A. Grieshaber, C. Ko, S.S. Grieshaber, I. Ji, T.H. Ji, Follicle-stimulating hormone-responsive cytoskeletal genes in rat granulosa cells: class I beta-tubulin, tropomyosin-4, and kinesin heavy chain. Endocrinology 144, 29–39 (2003)CrossRefPubMed
7.
R. Sasson, A. Dantes, K. Tajima, A. Amsterdam, Novel genes modulated by FSH in normal and immortalized FSH-responsive cells: new insights into the mechanism of FSH action. FASEB J. 17, 1256–1266 (2003)CrossRefPubMed
8.
M. Tanaka, J.D. Hennebold, K. Miyakoshi, T. Teranishi, K. Ueno, E.Y. Adashi, The generation and characterization of an ovary-selective cDNA library. Mol. Cell. Endocrinol. 202, 67–69 (2003)PubMed
9.
S. Shimasaki, R.J. Zachow, D. Li, H. Kim, S. Iemura, N. Ueno, K. Sampath, R.J. Chang, G.F. Erickson, A functional bone morphogenetic protein system in the ovary. Proc. Natl. Acad. Sci. USA 96, 7282–7287 (1999)CrossRefPubMed
10.
N. Yao, C.L. Lu, J.J. Zhao, H.F. Xia, D.G. Sun, X.Q. Shi, C. Wang, D. Li, Y. Cui, X. Ma, A network of miRNAs expressed in the ovary are regulated by FSH. Front Biosci. 14, 3239–3245 (2009)CrossRefPubMed
11.
M. Lagos-Quintana, R. Rauhut, W. Lendeckel, T. Tuschl, Identification of novel genes coding for small expressed RNAs. Science 294, 853–858 (2001)CrossRefPubMed
12.
A.M. Cheng, M.W. Byrom, J. Shelton, L.P. Ford, Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Res. 33, 1290–1297 (2005)CrossRefPubMed
13.
S. Vasudevan, Y. Tong, J.A. Steitz, Switching from repression to activation: microRNAs can up-regulate translation. Science 318, 1931–1934 (2007)CrossRefPubMed
14.
A. Cohen, M. Shmoish, L. Levi, U. Cheruti, B. Levavi-Sivan, E. Lubzens, Alterations in micro-ribonucleic acid expression profiles reveal a novel pathway for estrogen regulation. Endocrinology 149, 1687–1696 (2008)CrossRefPubMed
15.
S.D. Fiedler, M.Z. Carletti, X. Hong, L.K. Christenson, Hormonal regulation of MicroRNA expression in periovulatory mouse mural granulosa cells. Biol. Reprod. 79, 1030–1037 (2008)CrossRefPubMed
16.
T. Yuen, F. Ruf, T. Chu, S.C. Sealfon, Microtranscriptome regulation by gonadotropin-releasing hormone. Mol. Cell. Endocrinol. 302, 12–17 (2009)CrossRefPubMed
17.
G. Stefani, F.J. Slack, Small non-coding RNAs in animal development. Nat. Rev. Mol. Cell. Biol. 9, 219–230 (2008)CrossRefPubMed
18.
M. Otsuka, M. Zheng, M. Hayashi, J.D. Lee, O. Yoshino, S. Lin, J. Han, Impaired microRNA processing causes corpus luteum insufficiency and infertility in mice. J. Clin. Invest. 118, 1944–1954 (2008)CrossRefPubMed
19.
S. Ro, R. Song, C. Park, H. Zheng, K.M. Sanders, W. Yan, Cloning and expression profiling of small RNAs expressed in the mouse ovary. RNA 13, 2366–2380 (2007)CrossRefPubMed
20.
N.J. Martinez, M.C. Ow, M.I. Barrasa, M. Hammell, R. Sequerra, L. Doucette-Stamm, F.P. Roth, V.R. Ambros, A.J. Walhout, A C. elegans genome-scale microRNA network contains composite feedback motifs with high flux capacity. Genes Dev. 22, 2535–2549 (2008)CrossRefPubMed
21.
S.M. Johnson, S.Y. Lin, F.J. Slack, The time of appearance of the C. elegans let-7 microRNA is transcriptionally controlled utilizing a temporal regulatory element in its promoter. Dev. Biol. 259, 364–379 (2003)CrossRefPubMed
22.
K.A. O’Donnell, E.A. Wentzel, K.I. Zeller, C.V. Dang, J.T. Mendell, c-Myc-regulated microRNAs modulate E2F1 expression. Nature 435, 839–843 (2005)CrossRefPubMed
23.
Y. Sylvestre, V. De Guire, E. Querido, U.K. Mukhopadhyay, V. Bourdeau, F. Major, G. Ferbeyre, P. Chartrand, An E2F/miR-20a autoregulatory feedback loop. J Biol. Chem. 282, 2135–2143 (2007)CrossRefPubMed
24.
M. Yamakuchi, C.J. Lowenstein, MiR-34, SIRT1 and p53: the feedback loop. Cell Cycle 8, 712–715 (2009)PubMed
25.
B.P. Lewis, C.B. Burge, D.P. Bartel, Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120, 15–20 (2005)CrossRefPubMed
26.
P.K. Rao, R.M. Kumar, M. Farkhondeh, S. Baskerville, H.F. Lodish, Myogenic factors that regulate expression of muscle-specific microRNAs. Proc. Natl. Acad. Sci. USA 103, 8721–8726 (2006)CrossRefPubMed
27.
S. Marton, M.R. Garcia, C. Robello, H. Persson, F. Trajtenberg, O. Pritsch, C. Rovira, H. Naya, G. Dighiero, A. Cayota, Small RNAs analysis in CLL reveals a deregulation of miRNA expression and novel miRNA candidates of putative relevance in CLL pathogenesis. Leukemia 22, 330–338 (2008)CrossRefPubMed
28.
C.A. Gebeshuber, K. Zatloukal, J. Martinez, miR-29a suppresses tristetraprolin, which is a regulator of epithelial polarity and metastasis. EMBO Rep. 10, 400–405 (2009)CrossRefPubMed
29.
J. Wang, R. Xu, F. Lin, S. Zhang, G. Zhang, S. Hu, Z. Zheng, MicroRNA: novel regulators involved in the remodeling and reverse remodeling of the heart. Cardiology 113, 81–88 (2009)CrossRefPubMed
30.
A. He, L. Zhu, N. Gupta, Y. Chang, F. Fang, Overexpression of micro ribonucleic acid 29, highly up-regulated in diabetic rats, leads to insulin resistance in 3T3–L1 adipocytes. Mol. Endocrinol. 21, 2785–2794 (2007)CrossRefPubMed
31.
X. Tang, L. Muniappan, G. Tang, S. Ozcan, Identification of glucose-regulated miRNAs from pancreatic beta cells reveals a role for miR-30d in insulin transcription. RNA 15, 287–293 (2009)CrossRefPubMed
32.
A.E. Williams, S.A. Moschos, M.M. Perry, P.J. Barnes, M.A. Lindsay, Maternally imprinted microRNAs are differentially expressed during mouse and human lung development. Dev. Dyn. 236, 572–580 (2007)CrossRefPubMed
33.
M. Hunzicker-Dunn, E.T. Maizels, FSH signaling pathways in immature granulosa cells that regulate target gene expression: branching out from protein kinase A. Cell. Signal. 18, 1351–1359 (2006)CrossRefPubMed
34.
C.K. Sites, B. Kessel, A.R. LaBarbera, Adhesion proteins increase cellular attachment, follicle-stimulating hormone receptors, and progesterone production in cultured porcine granulosa cells. Proc. Soc. Exp. Biol. Med. 212, 78–83 (1996)PubMed
35.
K. Nakano, I. Naito, R. Momota, Y. Sado, H. Hasegawa, Y. Ninomiya, A. Ohtsuka, The distribution of type IV collagen alpha chains in the mouse ovary and its correlation with follicular development. Arch. Histol. Cytol. 70, 243–253 (2007)CrossRefPubMed
36.
N.B. Gilula, M.L. Epstein, W.H. Beers, Cell-to-cell communication and ovulation. A study of the cumulus-oocyte complex. J. Cell Biol. 78, 58–75 (1978)CrossRefPubMed
37.
C. Kohler, C.B. Villar, Programming of gene expression by Polycomb group proteins. Trends Cell Biol. 18, 236–243 (2008)CrossRefPubMed
38.
L.A. Boyer, K. Plath, J. Zeitlinger, T. Brambrink, L.A. Medeiros, T.I. Lee, S.S. Levine, M. Wernig, A. Tajonar, M.K. Ray, G.W. Bell, A.P. Otte, M. Vidal, D.K. Gifford, R.A. Young, R. Jaenisch, Polycomb complexes repress developmental regulators in murine embryonic stem cells. Nature 441, 349–353 (2006)CrossRefPubMed
39.
P.F. Terranova, F. Garza, Relationship between the preovulatory luteinizing hormone (LH) surge and androstenedione synthesis of preantral follicles in the cyclic hamster: detection by in vitro responses to LH. Biol. Reprod. 29, 630–636 (1983)CrossRefPubMed
40.
M. Ashburner, C.A. Ball, J.A. Blake, D. Botstein, H. Butler, J.M. Cherry, A.P. Davis, K. Dolinski, S.S. Dwight, J.T. Eppig, M.A. Harris, D.P. Hill, L. Issel-Tarver, A. Kasarskis, S. Lewis, J.C. Matese, J.E. Richardson, M. Ringwald, G.M. Rubin, G. Sherlock, Gene ontology: tool for the unification of biology. The gene ontology consortium. Nat. Genet. 25, 25–29 (2000)CrossRefPubMed
41.
M. Kanehisa, S. Goto, M. Hattori, K.F. Aoki-Kinoshita, M. Itoh, S. Kawashima, T. Katayama, M. Araki, M. Hirakawa, From genomics to chemical genomics: new developments in KEGG. Nucleic Acids Res. 34, D354–D357 (2006)CrossRefPubMed
Metadata
Title
Follicle-stimulating hormone regulation of microRNA expression on progesterone production in cultured rat granulosa cells
Authors
Nan Yao
Bai-Qing Yang
Yu Liu
Xin-Yu Tan
Cai-Ling Lu
Xiao-Hua Yuan
Xu Ma
Publication date
01-10-2010
Publisher
Springer US
Published in
Endocrine / Issue 2/2010
Print ISSN: 1355-008X
Electronic ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-010-9345-1

Other articles of this Issue 2/2010

Endocrine 2/2010 Go to the issue

Original Article

Selective α1-adrenoceptor antagonist (controlled release tablets) in preoperative management of pheochromocytoma

Erratum

Erratum to: Preliminary biochemical characterization of the novel, non-AT1, non-AT2 angiotensin binding site from the rat brain

Original Article

Haptoglobin phenotype in women with preeclampsia

Original Article

Oxidative DNA damage: the thyroid hormone-mediated effects of insulin on liver tissue

Original Article

A comparison of antineutrophil cytoplasmic antibody prevalence in patients treated and untreated for hyperthyroidism

Original Article

Atypical induction of the unfolded protein response by mifepristone
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits