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Molecular Cancer
Published in: Molecular Cancer 1/2008

Open Access 01-12-2008 | Research

Potential role of miR-9 and miR-223 in recurrent ovarian cancer

Authors: Alexandros Laios, Sharon O'Toole, Richard Flavin, Cara Martin, Lynne Kelly, Martina Ring, Stephen P Finn, Ciara Barrett, Massimo Loda, Noreen Gleeson, Tom D'Arcy, Eamonn McGuinness, Orla Sheils, Brian Sheppard, John O' Leary

Published in: Molecular Cancer | Issue 1/2008

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Abstract

Background

MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression by binding to target mRNAs. miRNAs have not been comprehensively studied in recurrent ovarian cancer, yet an incurable disease.

Results

Using real-time RT-PCR, we obtained distinct miRNA expression profiles between primary and recurrent serous papillary ovarian adenocarcinomas (n = 6) in a subset of samples previously used in a transcriptome approach. Expression levels of top dysregulated miRNA genes, miR-223 and miR-9, were examined using TaqMan PCR in independent cohorts of fresh frozen (n = 18) and FFPE serous ovarian tumours (n = 22). Concordance was observed on TaqMan analysis for miR-223 and miR-9 between the training cohort and the independent test cohorts. Target prediction analysis for the above miRNA "recurrent metastatic signature" identified genes previously validated in our transcriptome study. Common biological pathways well characterised in ovarian cancer were shared by miR-9 and miR-223 lists of predicted target genes. We provide strong evidence that miR-9 acts as a putative tumour suppressor gene in recurrent ovarian cancer. Components of the miRNA processing machinery, such as Dicer and Drosha are not responsible for miRNA deregulation in recurrent ovarian cancer, as deluded by TaqMan and immunohistochemistry.

Conclusion

We propose a miRNA model for the molecular pathogenesis of recurrent ovarian cancer. Some of the differentially deregulated miRNAs identified correlate with our previous transcriptome findings. Based on integrated transcriptome and miRNA analysis, miR-9 and miR-223 can be of potential importance as biomarkers in recurrent ovarian cancer.
Appendix
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Metadata
Title
Potential role of miR-9 and miR-223 in recurrent ovarian cancer
Authors
Alexandros Laios
Sharon O'Toole
Richard Flavin
Cara Martin
Lynne Kelly
Martina Ring
Stephen P Finn
Ciara Barrett
Massimo Loda
Noreen Gleeson
Tom D'Arcy
Eamonn McGuinness
Orla Sheils
Brian Sheppard
John O' Leary
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2008
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/1476-4598-7-35

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