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Open Access 01-12-2010 | Research

Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection

Authors: Lyda Osorio, Meleny Ramirez, Anilza Bonelo, Luis A Villar, Beatriz Parra

Published in: Virology Journal | Issue 1/2010

Abstract

Background

We compared the diagnostic accuracy and reproducibility of commercially available NS1-based dengue tests and explored factors influencing their sensitivities.

Methods

Paired analysis of 310 samples previously characterized as positive (n = 218) and negative (n = 92) for viral isolation and/or RT-PCR and/or IgM seroconversion. Masked samples were tested by two observers with Platelia™ Dengue NS1 Ag, second generation Pan-E™ Dengue Early ELISA, SD Dengue NS1 Ag ELISA, Dengue NS1 Ag STRIP™, and SD BIOLINE™ Dengue Duo (NS1/IgM/IgG).

Results

SD BIOLINE™ NS1/IgM/IgG had the highest sensitivity (80.7% 95%CI 75-85.7) with likelihood ratios of 7.4 (95%CI 4.1-13.8) and 0.21 (95%CI 0.16-0.28). The ELISA-format tests showed comparable sensitivities; all below 75%. STRIP™ and SD NS1 had even lower sensitivities (<65%). The sensitivities significantly decreased in samples taken after 3 days of fever onset, in secondary infections, viral serotypes 2 and 4, and severe dengue. Adding IgM or IgG to SD NS1 increased its sensitivity in all these situations.

Conclusions

The simultaneous detection of NS1/IgM/IgG would be potentially useful for dengue diagnosis in both endemic and non endemic areas. A negative result does not rule out dengue. Further studies are required to assess the performance and impact of early laboratory diagnosis of dengue in the routine clinical setting.

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Title: Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection
Authors: Lyda Osorio
Meleny Ramirez
Anilza Bonelo
Luis A Villar
Beatriz Parra
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-7-361

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Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection

Abstract

Background

Methods

Results

Conclusions

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Abstract

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