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Virology Journal
Published in: Virology Journal 1/2008

Open Access 01-12-2008 | Research

Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea

Authors: Stacy R Finkbeiner, Carl D Kirkwood, David Wang

Published in: Virology Journal | Issue 1/2008

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Abstract

Background

Astroviruses infect a variety of mammals and birds and are causative agents of diarrhea in humans and other animal hosts. We have previously described the identification of several sequence fragments with limited sequence identity to known astroviruses in a stool specimen obtained from a child with acute diarrhea, suggesting that a novel virus was present.

Results

In this study, the complete genome of this novel virus isolate was sequenced and analyzed. The overall genome organization of this virus paralleled that of known astroviruses, with 3 open reading frames identified. Phylogenetic analysis of the ORFs indicated that this virus is highly divergent from all previously described animal and human astroviruses. Molecular features that are highly conserved in human serotypes 1–8, such as a 3'NTR stem-loop structure and conserved nucleotide motifs present in the 5'NTR and ORF1b/2 junction, were either absent or only partially conserved in this novel virus.

Conclusion

Based on the analyses described herein, we propose that this newly discovered virus represents a novel species in the family Astroviridae. It has tentatively been named Astrovirus MLB1.
Appendix
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Metadata
Title
Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea
Authors
Stacy R Finkbeiner
Carl D Kirkwood
David Wang
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2008
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-5-117

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