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Virology Journal

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Open Access 01-12-2014 | Short report

PCR detection and analyzis of potentially zoonotic Hepatitis E virus in French rats

Authors: Frederik Widén, Florence Ayral, Marc Artois, Ann-Sophie Olofson, Jay Lin

Published in: Virology Journal | Issue 1/2014

Abstract

Background

Hepatitis E virus has been detected in a wide range of animals. While Genotypes 1-2 of this virus infect only humans, 3-4 can spread from animals to humans and cause sporadic cases of human disease. Pig, and possibly also rats, may act as a reservoir for virus. From a public health perspective it is important to clarify the role of rats for infection of humans. Rats often live close to humans and are therefore of special interest to public health. Rats live of waste and inside the sewage system and may become infected. Reports of hepatitis E virus in rats have been published but not from France. The possibility that rats in an urban area in France were Hepatitis E virus infected, with which type and relationship to other strains was investigated. This study provides information important to public health and better understanding the occurrence of hepatitis E virus in the environment.

Eighty one rats (Rattus Norvegicus) were captured, euthanized, sampled (liver and faeces) and analyzed by real-time RT-PCR’s, one specific for Hepatitis E virus in rats and one specific for genotype 1-4 that that is known to infect humans. Positive samples were analyzed by a nested broad spectrum RT-PCR, sequenced and compared with sequences in Genbank.

Findings

Twelve liver and 11 faeces samples out of 81 liver and 81 faeces samples from 81 captured rats were positive in the PCR specific for Hepatitis E virus in rats and none in the PCR specific for genotype 1-4. Comparison by nucleotide BLAST showed a maximum of 87% similarity to Hepatitis E virus previously detected in rats and significantly less to genotype 1-4.

Conclusions

This is the first study demonstrating that rats in France carries hepatitis E virus and provide information regarding its relation to other virus strains previously detected in rats and other host animals world-wide. Genotype 1-4 was not detected.

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Cleaveland S, Laurenson MK, Taylor LH: Diseases of humans and their domestic mammals: pathogen characteristics, host range and the risk of emergence. Philos Trans R Soc Lond B Biol Sci. 2001, 356: 991-999. 10.1098/rstb.2001.0889.PubMedPubMedCentralCrossRef

Jones KE, Patel NG, Levy MA, Storeygard A, Balk D, Gittleman JL, Daszak P: Global trends in emerging infectious diseases. Nature. 2008, 451: 990-993. 10.1038/nature06536.PubMedCrossRef

Purcell RH, Emerson SU: Hidden danger: the raw facts about hepatitis E virus. J Infect Dis. 2010, 202: 819-821. 10.1086/655900.PubMedPubMedCentralCrossRef

Arends JE, Ghisetti V, Irving W, Dalton HR, Izopet J, Hoepelman AIM, Salmon D: Hepatitis E: an emerging infection in high income countries. J Clin Virol. 2014, 59: 81-88. 10.1016/j.jcv.2013.11.013.PubMedCrossRef

Lu L, Li C, Hagedorn CH: Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev Med Virol. 2006, 16: 5-36. 10.1002/rmv.482.PubMedCrossRef

Boutrouille A, Bakkali-Kassimi L, Cruciére C, Pavio N: Prevalence of Anti-Hepatitis E Virus antibodies in French blood donors. J Clin Microb. 2007, 45: 2009-2010. 10.1128/JCM.00235-07.CrossRef

Rose N, Lunazzi A, Dorenlor V, Merbah T, Eono F, Eloit M, Madec F, Pavio N: High prevalence of Hepatitis E virus in French domestic pigs. Comp Immunol Microbiol Infect Dis. 2011, 34: 419-427. 10.1016/j.cimid.2011.07.003.PubMedCrossRef

Payne A, Rossi S, Lacour SA, Vallée I, Garin-Bastuji B, Simon G, Hervé S, Pavio N, Richomme C, Dunoyer C, Bronner A, Hars J: Bilan sanitaire du sanglier vis-à-vis de la trichinellose, de la maladie d’Aujeszky, de la brucellose, de l’hépatite E et des virus influenza porcins en France. Bulletin Epidemiologique Santé Animal – Alimentation. 2011, 44: 2-8.

Smith DB, Purdy MA, Simmonds P: Genetic variability and the classification of hepatitis E virus. J Virol. 2013, 87: 4161-4169. 10.1128/JVI.02762-12.PubMedPubMedCentralCrossRef

10.

Lin J, Norder H, Uhlhorn H, Belák S, Widén F: Novel hepatitis E like virus found in Swedish moose. J Gen Virol. 2014, 95: 557-570. 10.1099/vir.0.059238-0.PubMedPubMedCentralCrossRef

11.

Izopet J, Dubois M, Bertagnoli S, Lhomme S, Marchandeau S, Boucher S, Kamar N, Abravanel F, Guérin JL: Hepatitis E virus strains in rabbits and evidence of a closely related strain in humans, France. Emerg Infect Dis. 2012, 18: 1274-1281.PubMedPubMedCentralCrossRef

12.

Raj VS, Smits SL, Pas SD, Provacia LBV, Moorman-Roest H, Osterhaus ADME, Haagmans BL: Novel Hepatitis E Virus in Ferrets, the Netherlands. Emerg Infect Dis. 2012, 18: 1369-1370. 10.3201/eid1808.111659.PubMedPubMedCentralCrossRef

13.

Johne R, Heckel G, Plenge-Bonig A, Kindler E, Maresch C, Reetz J, Schielke A, Ulrich RG: Novel hepatitis E virus genotype in Norway rats, Germany. Emerg Infect Dis. 2010, 16: 1452-1455. 10.3201/eid1609.100444.PubMedPubMedCentralCrossRef

14.

Huang FF, Sun ZF, Emerson SU, Purcell RH, Shivaprasad HL, Pierson FW, Toth TE, Meng XJ: Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV. J Gen Virol. 2004, 85: 1609-1618. 10.1099/vir.0.79841-0.PubMedCrossRef

15.

Batts W, Yun S, Hedrick R, Winton J: A novel member of the family Hepeviridae from cutthroat trout (Oncorhynchus clarkii). Virus Res. 2011, 158: 116-123. 10.1016/j.virusres.2011.03.019.PubMedCrossRef

16.

Lack JB, Volk K, Van Den Bussche RA: Hepatitis E virus genotype 3 in wild rats, United States. Emerg Infect Dis. 2012, 18: 1268-1273.PubMedPubMedCentralCrossRef

17.

Johne R, Dremsek P, Kindler E, Schielke A, Plenge-Bonig A, Gregersen H, Wessels U, Schmidt K, Rietschel W, Groschup MH, Guenther S, Heckel G, Ulrich RG: Rat hepatitis E virus: Geographical clustering within Germany and serological detection in wild Norway rats (Rattus norvegicus). Infect Genet Evol: J Mol Epidemiol Evol Genet Infect Dis. 2012, 12: 947-956. 10.1016/j.meegid.2012.02.021.CrossRef

18.

Cossaboom CM, Córdoba L, Sanford BJ, Piñeyro P, Kenney SP, Dryman BA, Wang Y, Meng XJ: Cross-species infection of pigs with a novel rabbit, but not rat, strain of hepatitis E virus isolated in the United States. J Gen Virol. 2012, 93: 1687-1695. 10.1099/vir.0.041509-0.PubMedPubMedCentralCrossRef

19.

Dremsek P, Wenzel JJ, Johne R, Ziller M, Hofmann J, Groschup MH, Werdermann S, Mohn U, Dorn S, Motz M, Mertens M, Jilg W, Ulrich RG: Seroprevalence study in forestry workers from eastern Germany using novel genotype 3- and rat hepatitis E virus-specific immunoglobulin G ELISAs. Med Microbiol Immunol. 2012, 201: 189-200. 10.1007/s00430-011-0221-2.PubMedCrossRef

20.

Li TC, Ami Y, Suzaki Y, Yasuda SP, Yoshimatsu K, Arikawa J, Takeda N, Takaji W: Characterization of full genome of rat hepatitis E virus strain from Vietnam. Emerg Infect Dis. 2013, 19: 115-118. 10.3201/eid1901.121007.PubMedPubMedCentralCrossRef

21.

Wolf S, Reetz J, Johne R, Heiberg AC, Petri S, Kanig H, Ulrich RG: The simultaneous occurrence of human norovirus and hepatitis E virus in a Norway rat (Rattus norvegicus). Arch Virol. 2013, 158: 1575-1578. 10.1007/s00705-013-1646-2.PubMedCrossRef

22.

Li W, Guan D, Su J, Takeda N, Wakita T, Li TC, Ke CW: High prevalence of rat hepatitis E virus in wild rats in China. Vet Microbiol. 2013, 165: 275-280. 10.1016/j.vetmic.2013.03.017.PubMedCrossRef

23.

Mulyanto , Suparyatmo JB, Andayani IG, Khalid , Takahashi M, Ohnishi H, Jirintai S, Nagashima S, Nishizawa T, Okamoto H: Marked genomic heterogeneity of rat hepatitis E virus strains in Indonesia demonstrated on a full-length genome analysis. Virus Res. 2014, 179: 102-112.PubMedCrossRef

24.

Jothikumar N, Cromeans TL, Robertson BH, Meng XJ, Hill VR: A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus. J Virol Methods. 2006, 131: 65-71. 10.1016/j.jviromet.2005.07.004.PubMedCrossRef

25.

Johne R, Plenge-Bönig A, Hess M, Ulrich RG, Reetz J, Schielke A: Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR. J Gen Virol. 2010, 91: 750-758. 10.1099/vir.0.016584-0.PubMedCrossRef

Title: PCR detection and analyzis of potentially zoonotic Hepatitis E virus in French rats
Authors: Frederik Widén
Florence Ayral
Marc Artois
Ann-Sophie Olofson
Jay Lin
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2014
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-11-90

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