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Journal of Translational Medicine
Published in: Journal of Translational Medicine 1/2014

Open Access 01-12-2014 | Research

Prednisolone has a positive effect on the kidney but not on the liver of brain dead rats: a potencial role in complement activation

Authors: Rolando Rebolledo, Bo Liu, Mohammed Z Akhtar, Petra J Ottens, Jian-ning Zhang, Rutger J Ploeg, Henri GD Leuvenink

Published in: Journal of Translational Medicine | Issue 1/2014

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Abstract

Background

Contradictory evidence has been published on the effects of steroid treatments on the outcomes of kidney and liver transplantation from brain dead (BD) donors. Our study aimed to evaluate this disparity by investigating the effect of prednisolone administration on BD rats.

Methods

BD induction was performed in ventilated rats by inflating a Fogarty catheter placed in the epidural space. Prednisolone (22.5 mg/kg) was administered 30 min prior to BD induction. After four hours of determination of BD: serum, kidney and liver tissues samples were collected and stored. RT-qPCR, routine biochemistry and immunohistochemistry were performed.

Results

Prednisolone treatment reduced circulating IL-6 and creatinine plasma levels but not serum AST, ALT or LDH. Polymorphonuclear influx assessed by histology, and inflammatory gene expression were reduced in the kidney and liver. However, complement component 3 (C3) expression was decreased in kidney but not in liver. Gene expression of HSP-70, a cytoprotective protein, was down-regulated in the liver after treatment.

Conclusions

This study shows that prednisolone decreases inflammation and improves renal function, whilst not reducing liver injury. The persistence of complement activation and the negative effect on protective cellular mechanisms in the liver may explain the disparity between the effects of prednisolone on the kidney and liver of BD rats. The difference in the molecular and cellular responses to prednisolone administration may explain the contradictory evidence of the effects of prednisolone on different organ types from brain dead organ donors.
Appendix
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Metadata
Title
Prednisolone has a positive effect on the kidney but not on the liver of brain dead rats: a potencial role in complement activation
Authors
Rolando Rebolledo
Bo Liu
Mohammed Z Akhtar
Petra J Ottens
Jian-ning Zhang
Rutger J Ploeg
Henri GD Leuvenink
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Journal of Translational Medicine / Issue 1/2014
Electronic ISSN: 1479-5876
DOI
https://doi.org/10.1186/1479-5876-12-111

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