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Open Access 01-12-2014 | Research

Gene silencing of heparanase results in suppression of invasion and migration of hepatoma cells

Authors: Weiwei Dong, Huixia Zhao, Caihong Zhang, Paili Geng, Sarengaowa, Qiuwen Li, Jianhua Zhu, Guanghui Li, Shufang Zhang, Ming Ye, Wenhua Xiao

Published in: World Journal of Surgical Oncology | Issue 1/2014

Abstract

Background

This study investigated the effect of transcriptional gene silencing (TGS) of the heparanase gene on hepatoma SMCC-7721 cells.

Methods

SiRNAs targeting the promoter region and coding region of the heparanase gene were designed and synthesized. Then the siRNAs were transfected into hepatoma SMCC-7721 cells by nuclear transfection or cytoplasmic transfection. The expression of heparanase was detected by RT-PCR and Western blotting 48 h, 72 h and 96 h post-transfection. In addition, wound healing and invasion assays were performed to estimate the effect of TGS of the heparanase gene on the migration and invasion of hepatoma SMCC-7721 cells.

Results

Protein and mRNA expression of the heparanase gene were interfered with by TGS or post-transcriptional gene silencing (PTGS) 48 h after transfection. At 72 h post-transfection, the expression of the PTGS group of genes had recovered unlike the TGS group. At 96 h post-transfection, the expression of the heparanase gene had recovered in both the TGS group and PTGS group. Invasion and wound healing assays showed that both TGS and PTGS of the heparanase gene could inhibit invasion and migration of hepatoma SMCC-7721 cells, especially the TGS group.

Conclusions

TGS can effectively interfere with the heparanase gene to reduce the invasion and migration of hepatoma SMCC-7721 cells.

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Title: Gene silencing of heparanase results in suppression of invasion and migration of hepatoma cells
Authors: Weiwei Dong
Huixia Zhao
Caihong Zhang
Paili Geng
Sarengaowa
Qiuwen Li
Jianhua Zhu
Guanghui Li
Shufang Zhang
Ming Ye
Wenhua Xiao
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2014
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/1477-7819-12-85

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Gene silencing of heparanase results in suppression of invasion and migration of hepatoma cells

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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