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World Journal of Surgical Oncology
Published in: World Journal of Surgical Oncology 1/2014

Open Access 01-12-2014 | Research

Glucose-regulated protein 78 and heparanase expression in oral squamous cell carcinoma: correlations and prognostic significance

Authors: Fei Xia, Jin Cheng Xu, Pei Zhang, Yuan Yuan Zhang, Qian Wen Zhang, Zhen Hua Chao, Fang Wang

Published in: World Journal of Surgical Oncology | Issue 1/2014

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Abstract

Background

The aim of the present study was to investigate the expression of glucose-related protein 78 (GRP78) and heparanase (HPA) in oral squamous cell carcinoma (OSCC) and their relationship with clinicopathological parameters and potential implications for survival.

Methods

A total of 46 patients with OSCC and 10 normal individuals were recruited for the study. GRP78 and HPA expression were determined in the lesion tissues using immunohistochemical analysis. The correlation between GRP78 and HPA was assessed using the Spearman correlation analysis. The associations of GRP78 and HPA with clinicopathological characteristics and survival were examined using the x2-test, Kaplan–Meier, or Cox regression.

Results

Patients with OSCC showed a statistically significant higher prevalence of GRP78 and HPA expression than normal oral tissues. GRP78 and HPA expression was positively correlated with size, TNM stage, histological grade, lymphatic metastasis, and distant metastasis in OSCC patients. GRP78 expression was also positively correlated with HPA expression. Positive GRP78 and HPA expression was inversely correlated with survival in OSCC patients.

Conclusions

HPA expression was found to be positively correlated with GRP78 expression. GRP78 and HPA are biomarkers that may have the potential to guide the treatment of oral cancer patients.
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Literature
1.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D: Global cancer statistics. CA Cancer J Clin. 2011, 61: 69-90. 10.3322/caac.20107.CrossRefPubMed
2.
Warnakulasuriya S: Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 2009, 45: 309-316. 10.1016/j.oraloncology.2008.06.002.CrossRefPubMed
3.
Awada A, Aftimos PG: Targeted therapies of solid cancers: new options, new challenges. Curr Opin Oncol. 2013, 25: 296-304.PubMed
4.
5.
Zhang LH, Zhang X: Roles of GRP78 in physiology and cancer. J Cell Biochem. 2010, 110: 1299-1305. 10.1002/jcb.22679.CrossRefPubMed
6.
Hetz C: The unfolded protein response: controlling cell fate decisions under ER stress and beyond. Nat Rev Mol Cell Biol. 2012, 13: 89-102.PubMed
7.
Hetz C, Martinon F, Rodriguez D, Glimcher LH: The unfolded protein response: integrating stress signals through the stress sensor IRE1alpha. Physiol Rev. 2011, 91: 1219-1243. 10.1152/physrev.00001.2011.CrossRefPubMed
8.
Pu L, Huang Y, Li Y, Xu J, Jiang C, Liu H, Jiang Z: Small interfering RNA-mediated glucose-regulated protein 78 knockdown enhances chemosensitivity of breast cancer cells to cisplatin. Nan Fang Yi Ke Da Xue Xue Bao. 2013, 33: 44-47.PubMed
9.
Maddalo D, Neeb A, Jehle K, Schmitz K, Muhle-Goll C, Shatkina L, Walther TV, Bruchmann A, Gopal SM, Wenzel W, Ulrich AS, Cato AC: A peptidic unconjugated GRP78/BiP ligand modulates the unfolded protein response and induces prostate cancer cell death. PLoS One. 2012, 7: e45690-10.1371/journal.pone.0045690.PubMedCentralCrossRefPubMed
10.
Nasser NJ: Heparanase involvement in physiology and disease. Cell Mol Life Sci. 2008, 65: 1706-1715. 10.1007/s00018-008-7584-6.CrossRefPubMed
11.
Yan L, Yan K, Kun W, Xu L, Ma Q, Tang Y, Jiao W, Gu G, Fan Y, Xu Z: Berberine inhibits the migration and invasion of T24 bladder cancer cells via reducing the expression of heparanase. Tumour Biol. 2013, 34: 215-221. 10.1007/s13277-012-0531-z.CrossRefPubMed
12.
Chen Z, Zhu L, Li X, Tian H, Fang Y, Liu H, Li S, Li L, Yue W, Li W: Down-regulation of heparanase leads to the inhibition of invasion and proliferation of A549 cells in vitro and in vivo. Acta Biochim Biophys Sin. 2013, 45: 188-193. 10.1093/abbs/gms109.CrossRefPubMed
13.
Zhuang L, Scolyer RA, Lee CS, McCarthy SW, Cooper WA, Zhang XD, Thompson JF, Hersey P: Expression of glucose-regulated stress protein GRP78 is related to progression of melanoma. Histopathology. 2009, 54: 462-470. 10.1111/j.1365-2559.2009.03242.x.CrossRefPubMed
14.
Fernandez PM, Tabbara SO, Jacobs LK, Manning FC, Tsangaris TN, Schwartz AM, Kennedy KA, Patierno SR: Overexpression of the glucose-regulated stress gene GRP78 in malignant but not benign human breast lesions. Breast Cancer Res Treat. 2000, 59: 15-26. 10.1023/A:1006332011207.CrossRefPubMed
15.
Wang Q, He Z, Zhang J, Wang Y, Wang T, Tong S, Wang L, Wang S, Chen Y: Overexpression of endoplasmic reticulum molecular chaperone GRP94 and GRP78 in human lung cancer tissues and its significance. Cancer Detect Prev. 2005, 29: 544-551. 10.1016/j.cdp.2005.09.010.CrossRefPubMed
16.
Daneshmand S, Quek ML, Lin E, Lee C, Cote RJ, Hawes D, Cai J, Groshen S, Lieskovsky G, Skinner DG, Lee AS, Pinski J: Glucose-regulated protein GRP78 is up-regulated in prostate cancer and correlates with recurrence and survival. Hum Pathol. 2007, 38: 1547-1552. 10.1016/j.humpath.2007.03.014.CrossRefPubMed
17.
Shao Q, Ren P, Li Y, Peng B, Dai L, Lei N, Yao W, Zhao G, Li L, Zhang J: Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma. Int J Oncol. 2012, 41: 1061-1067.PubMedCentralPubMed
18.
Takahashi H, Wang JP, Zheng HC, Masuda S, Takano Y: Overexpression of GRP78 and GRP94 is involved in colorectal carcinogenesis. Histol Histopathol. 2011, 26: 663-71.PubMed
19.
Zheng HC, Takahashi H, Li XH, Hara T, Masuda S, Guan YF, Takano Y: Overexpression of GRP78 and GRP94 are markers for aggressive behavior and poor prognosis in gastric carcinomas. Hum Pathol. 2008, 39: 1042-9. 10.1016/j.humpath.2007.11.009.CrossRefPubMed
20.
Matsuo K, Gray MJ, Yang DY, Srivastava SA, Tripathi PB, Sonoda LA, Yoo EJ, Dubeau L, Lee AS, Lin YG: The endoplasmic reticulum stress marker, glucose-regulated protein-78 (GRP78) in visceral adipocytes predicts endometrial cancer progression and patient survival. Gynecol Oncol. 2013, 128: 552-559. 10.1016/j.ygyno.2012.11.024.PubMedCentralCrossRefPubMed
21.
Kuroda K, Horiguchi A, Asano T, Ito K, Asakuma J, Sato A, Yoshii H, Hayakawa M, Sumitomo M, Asano T: Glucose-regulated protein 78 positivity as a predictor of poor survival in patients with renal cell carcinoma. Urol Int. 2011, 87: 450-456. 10.1159/000330883.CrossRefPubMed
22.
John A, Robin TV, Mario GG, Salvatore VP, James B, Kenneth EY: Patterns of GRP78 and MTJ1 expression in primary cutaneous malignant melanoma. Mod Pathol. 2010, 23: 134-143. 10.1038/modpathol.2009.152.CrossRef
23.
Baraz L, Haupt Y, Elkin M, Peretz T, Vlodavsky I: Tumor suppressor p53 regulates heparanase gene expression. Oncogene. 2006, 25: 3939-3947. 10.1038/sj.onc.1209425.CrossRefPubMed
24.
Wu W, Pan C, Meng K, Zhao L, Du L, Liu Q, Lin R: Hypoxia activates heparanase expression in an NF-kappaB dependent manner. Oncol Rep. 2010, 23: 255-261.CrossRefPubMed
25.
Li Y, Liu H, Huang YY, Pu LJ, Zhang XD, Jiang CC, Jiang ZW: Suppression of endoplasmic reticulum stress-induced invasion and migration of breast cancer cells through the downregulation of heparanase. Int J Mol Med. 2013, 31: 1234-42.PubMed
26.
Shafat I, Pode D, Peretz T, Ilan N, Vlodavsky I, Nisman B: Clinical significance of urine heparanase in bladder cancer progression. Neoplasia. 2008, 10: 125-130. 10.1593/neo.07875.PubMedCentralCrossRefPubMed
27.
Zhang J, Yang J, Han X, Zhao Z, DU L, Yu T, Wang H: Overexpression of heparanase multiple antigenic peptide 2 is associated with poor prognosis in gastric cancer: potential for therapy. Oncol Lett. 2012, 4: 178-182.PubMedCentralPubMed
28.
Sato T, Yamaguchi A, Goi T, Hirono Y, Takeuchi K, Katayama K, Matsukawa S: Heparanase expression in human colorectal cancer and its relationship to tumor angiogenesis, hematogenous metastasis, and prognosis. J Surg Oncol. 2004, 87: 174-181. 10.1002/jso.20097.CrossRefPubMed
29.
Ren J, Liu H, Yan L, Tian S, Li D, Xu Z: Microvessel density and heparanase over-expression in clear cell renal cell cancer: correlations and prognostic significances. World J Surg Oncol. 2011, 9: 158-10.1186/1477-7819-9-158.PubMedCentralCrossRefPubMed
30.
Ilan N, Elkin M, Vlodavsky I: Regulation, function and clinical significance of heparanase in cancer metastasis and angiogenesis. Int J Biochem Cell Biol. 2006, 38: 2018-2039. 10.1016/j.biocel.2006.06.004.CrossRefPubMed
31.
Leiser Y, Abu-El-Naaj I, Sabo E, Akrish S, Ilan N, Ben-Izhak O, Peled M, Vlodavsky I: Prognostic value of heparanase expression and cellular localization in oral cancer. Head Neck. 2011, 33: 871-877. 10.1002/hed.21545.PubMedCentralCrossRefPubMed
32.
Jiang CC, Mao ZG, Avery-Kiejda KA, Wade M, Hersey P, Zhang XD: Glucose-regulated protein 78 antagonizes cisplatin and adriamycin in human melanoma cells. Carcinogenesis. 2009, 30: 197-204.CrossRefPubMed
Metadata
Title
Glucose-regulated protein 78 and heparanase expression in oral squamous cell carcinoma: correlations and prognostic significance
Authors
Fei Xia
Jin Cheng Xu
Pei Zhang
Yuan Yuan Zhang
Qian Wen Zhang
Zhen Hua Chao
Fang Wang
Publication date
01-12-2014
Publisher
BioMed Central
Published in
World Journal of Surgical Oncology / Issue 1/2014
Electronic ISSN: 1477-7819
DOI
https://doi.org/10.1186/1477-7819-12-121

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