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Malaria Journal
Published in: Malaria Journal 1/2012

Open Access 01-12-2012 | Methodology

An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Authors: Sandra Hasenkamp, Eleanor H Wong, Paul Horrocks

Published in: Malaria Journal | Issue 1/2012

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Abstract

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.
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Metadata
Title
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
Authors
Sandra Hasenkamp
Eleanor H Wong
Paul Horrocks
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2012
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/1475-2875-11-42

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