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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2014

Open Access 01-12-2014 | Research article

Effect of methanol extract of Dicranopteris linearis against carbon tetrachloride- induced acute liver injury in rats

Authors: Farah Hidayah Kamisan, Farhana Yahya, Siti Syariah Mamat, Mohamad Fauzi Fahmi Kamarolzaman, Norhafizah Mohtarrudin, Teh Lay Kek, Mohd Zaki Salleh, Mohd Khairi Hussain, Zainul Amiruddin Zakaria

Published in: BMC Complementary Medicine and Therapies | Issue 1/2014

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Abstract

Background

Dicranopteris linearis (family Gleicheniaceae) has been reported to possess anti-inflammatory and antioxidant activities but no attempt has been made to study its hepatoprotective potential. The aim of the present study was to determine the hepatoprotective effect of methanol extracts of D. linearis (MEDL) against carbon tetrachloride (CCl4)-induced acute liver injury in rats.

Methods

6 groups (n = 6) of rats received oral test solutions: 10% dimethyl sulfoxide (DMSO), 200 mg/kg silymarin, or MEDL (50, 250, and 500 mg/kg), once daily for 7 consecutive days, followed by hepatotoxicity induction with CCl4. Blood and liver were collected for biochemical and microscopic analysis. The extract was also subjected to antioxidant studies (e.g. 2, 2-diphenyl-1-picrylhydrazyl (DPPH)- and superoxide anion-radical scavenging assays, oxygen radical absorbance capacity (ORAC) test and total phenolic content (TPC) determination), phytochemical screening and HPLC analysis.

Results

Pretreatment with MEDL and silymarin significantly (P < 0.05) reduced the serum levels of AST, ALT and ALP, which were increased significantly (P < 0.05) in DMSO-pretreated group following treatment with CCl4. Histological analysis of liver tissues in groups pretreated with MEDL and silymarin showed mild necrosis and inflammation of the hepatocytes compared to the DMSO-pretreated group (negative control group). The MEDL showed higher DPPH- and superoxide anion-radical scavenging activity as well as high TPC and ORAC values indicating high antioxidant activity.

Conclusions

MEDL exerts hepatoprotective activity that could be partly contributed by its antioxidant activity and high phenolic content, and hence demands further investigation.
Appendix
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Literature
1.
Kumar CH, Ramesh A, Suresh Kumar JN, Mohammed Ishaq B: A review on hepatoprotective activity of medicinal plants. Int J Pharmaceut Sci Res. 2011, 23: 501-515.
2.
Ahsan R, Islam KM, Musaddik A, Haque E: Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in rats. Eur J Sci Res. 2009, 37 (2): 302-310.
3.
Chattopadhyay RR: Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: part II. J Ethnopharmacol. 2003, 89: 217-219. 10.1016/j.jep.2003.08.006.CrossRefPubMed
4.
Sargent S: Liver Diseases: An Essential Guide for Nurses and Health Care Professionals. 2009, United Kingdom: John Wiley & Sons, 352-CrossRef
5.
WHO: Guidelines on the conservation of medicinal plants. 1993, Gland, Switzerland: The International Union for Conservation of Nature and Natural Resources (IUCN), 1-
6.
White CP, Hirsch G, Patel S, Adams F, Peltekian KM: Complementary and alternative medicine use by patients chronically infected with hepatitis C virus. Can J Gastroenterol. 2007, 21 (9): 589-595.CrossRefPubMedPubMedCentral
7.
Bhawna S, Kumar SU: Hepatoprotective activity of some indigenous plants. Int J Pharm Tech Res. 2009, 4: 1330-1334.
8.
Babu BH, Shylesh BS, Padikkala J: Antioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia. 2001, 72: 272-277. 10.1016/S0367-326X(00)00300-2.CrossRefPubMed
9.
Bhoopat L, Srichairatanakool S, Kanjanapothi D, Taesotikul T, Thananchai H, Bhoopat T: Hepatoprotective effects of lychee (Litchi chinensis Sonn.): a combination of antioxidant and anti-apoptotic activities. J Ethnopharmacol. 2011, 136 (1): 55-66. 10.1016/j.jep.2011.03.061.CrossRefPubMed
10.
Fakurazi S, Sharifudin SA, Arulselvan P: Moringa oleifera hydroethanolic extracts effectively alleviate acetaminophen-induced hepatotoxicity in experimental rats through their antioxidant nature. Molecules. 2012, 17 (7): 8334-8350.CrossRefPubMed
11.
Derus ARM: Pengenalan dan Penggunaan Herba Ubatan. 1998, Kuala Lumpur: Multiple triple vision, 75-
12.
Zakaria ZA, Ghani ZD, Nor RN, Gopalan HK, Sulaiman MR, Jais AM, Somchit MN, Kader AA, Ripin J: Antinociceptive, anti-inflammatory, and antipyretic properties of an aqueous extract of Dicranopteris linearis leaves in experimental animal models. J Nat Med. 2008, 62 (2): 179-187. 10.1007/s11418-007-0224-x.CrossRefPubMed
13.
Chin WY: Ferns and fern allies. The Encyclopedia of Malaysia: plants. Edited by: Soepadmo E. 1998, Singapore: Didier Millet, 44-
14.
Hussaini J, Othman NA, Abdulla MA, Majid NA, Faroq HM, Ismail S: Gastroprotective effects of Dicranopteris linearis leaf extract against ethanol- induced gastric mucosal injury in rats. Sci Res Essays. 2012, 7 (18): 1761-1767.
15.
Zakaria ZA: Free radical scavenging of some plants in Malaysia. Iranian J Pharmacol Ther. 2007, 6: 87-91.
16.
Lai HY, Lim YY: Evaluation of antioxidant activities of the methanolic extracts of selected ferns in Malaysia. Int J Environ Sci Tech. 2011, 2 (6): 442-447.
17.
Zakaria ZA, Mohamed AM, Mohd Jamil NS, Rofiee MS, Fatimah CA, Mat Jais AM, Sulaiman MR, Somchit MN: In vitro anticancer activity of various extracts of the Malaysian, available but neglected, plants (Muntingia Calabura And Dicranopteris Linearis) against Mcf-7 And Ht-29 cancer cell lines. E–Pharmanexus. 2008, 1 (1): 10-17.
18.
Sadasivan S, Latha PG, Sasikumar JM, Rajashekaran S, Shyamal S, Shine VJ: Hepatoprotective studies on Hedyotis corymbosa (L.) Lam. J Ethnopharmacol. 2006, 106: 245-249. 10.1016/j.jep.2006.01.002.CrossRefPubMed
19.
Dash DK, Yeligar VC, Nayak SS, Ghosh T, Rajalingam D, Sengupta P: Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res. 2007, 6: 755-765.CrossRef
20.
Ikhiri K, Boureima D, Dan-Kouloudo D: Chemical screening of medicinal plants used in the traditional pharmacopoeia of Niger. Int J Pharmacog. 1992, 30: 251-262. 10.3109/13880209209054009.CrossRef
21.
Yahya F, Mamat SS, Kamarolzaman MFF, Seyedan AA, Jakius KF, Mahmood ND, Shahril MS, Suhaili Z, Mohtarrudin N, Susanti D, Somchit MN, Teh LK, Salleh MZ, Zakaria ZA: Hepatoprotective Activity of Methanolic Extracts of Leaves of Bauhinia purpurea in Paracetamol-induced Hepatic Damage in Rats. Evid-Based Compliment Alternat Med. 2012, 2012: 636580-10.1155/2013/636580
22.
Singleton VL, Rossi JA: “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents”. Am J Enol Vitic. 1965, 16: 144-158.
23.
Blois MS: Antioxidant determinations by the use of a stable free radical. Nature. 1958, 18: 1199-1200.CrossRef
24.
Liu F, Ooi VE, Chang ST: Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 1997, 60: 763-771. 10.1016/S0024-3205(97)00004-0.CrossRefPubMed
25.
Huang D, Ou B, Hampsch- Woodil M, Flanagan JA, Prior RL: High throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem. 2002, 5: 4437-4444.CrossRef
26.
El-Beshbishy HA, Mohamadin A, Nagy A, Abdel-Naim A: Amelioration of tamoxifen-induced liver injury in rats by grape seed extract, black seed extract and curcumin. Indian J Exp Biol. 2010, 48: 280-288.PubMed
27.
Wu Y, Yang L, Wang F, Wu X, Zhou C, Shi S, Mo J, Zhao Y: Hepatoprotective and antioxidative effects of total phenolics from Laggera pterodonta on chemical-induced injury in primary cultured neonatal rat hepatocytes. Food Chem Toxicol. 2007, 45 (8): 1349-1355. 10.1016/j.fct.2007.01.011.CrossRefPubMed
28.
Hsiao G, Shen MY, Lin KH, Lan MH, Wu LY, Chou DS, Lin CH, Su CH, Sheu JR: Antioxidative and hepatoprotective effects of Antrodia camphorata extract. J Agric Food Chem. 2003, 51 (11): 3302-3308. 10.1021/jf021159t.CrossRefPubMed
29.
Jain A, Soni M, Deb L, Jain A, Rout SP, Gupta VB, Krishna KL: Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves. J Ethnopharmacol. 2008, 115 (1): 61-66. 10.1016/j.jep.2007.09.009.CrossRefPubMed
30.
Ranawat L, Bhatt J, Patel J: Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylum armatum DC in CCl4-induced hepatic damage in rats. J Ethnopharmacol. 2010, 127 (3): 777-780. 10.1016/j.jep.2009.10.019.CrossRefPubMed
31.
Ramaiah SK: A toxicologist guide to the diagnostic interpretation of hepatic biochemical parameters. Food Chem Toxicol. 2007, 45 (4): 1551-1557.CrossRefPubMed
32.
Shyamal S, Latha PG, Shine VJ, Suja SR, Rajasekharan S, Devi TG: Hepatoprotective effects of Pittosporum neelgherrense Wight&Arn., a popular Indian ethnomedicine. J Ethnopharmacol. 2006, 107: 151-155. 10.1016/j.jep.2006.02.018.CrossRefPubMed
33.
Panteghini M: Aspartate aminotransferase isoenzymes. Clin Biochem. 1990, 23 (4): 311-319. 10.1016/0009-9120(90)80062-N.CrossRefPubMed
34.
Ahmed MB, Khater MR: Evaluation of the protective potential of Ambrosia maritime extract on acetaminophen-induced liver damage. J Ethnopharmacol. 2001, 75: 169-174. 10.1016/S0378-8741(00)00400-1.CrossRefPubMed
35.
Naik SR, Panda VS: Antioxidant and hepatoprotective effects of Ginkgo biloba phytosomes in carbon tetrachloride-induced liver injury in rodents. Liver Int. 2007, 27 (3): 393-399. 10.1111/j.1478-3231.2007.01463.x.CrossRefPubMed
36.
Gao J, Sun C-R, Yang J-H, Shi J-M, Du Y-G, Zhang Y-Y, Li J-H, Wan H-T: Evaluation of the hepatoprotective and antioxidant activities of Rubus parvifolius L. J Zhejiang Univ Sci B. 2011, 12 (2): 135-142. 10.1631/jzus.B1000117.CrossRefPubMedPubMedCentral
37.
Korekar G, Stobdan T, Arora R, Yadav A, Singh SB: Antioxidant capacity and phenolics content of apricot (Prunus armeniaca L.) kernel as a function of genotype. Plant Foods Hum Nutr. 2011, 66 (4): 376-383. 10.1007/s11130-011-0246-0.CrossRefPubMed
38.
Stanković MS: Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci. 2011, 33 (2011): 63-72.
39.
Sone Y, Moon JK, Mai TT, Thu NN, Asano E, Yamaguchi K, Otsuka Y, Shibamoto T: Antioxidant/anti-inflammatory activities and total phenolic content of extracts obtained from plants grown in Vietnam. J Sci Food Agric. 2011, 91: 2259-2264.PubMed
40.
Alhakmani F, Kumar S, Khan SA: Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed. 2013, 3: 623-627. 10.1016/S2221-1691(13)60126-4.CrossRefPubMedPubMedCentral
41.
Adetutu A, Owoade AO: Hepatoprotective and antioxidant effect of Hibiscus Polyphenol Rich Extract (HPE) against carbon tetrachloride (CCl4) –induced damage in rats. Br J Medic Med Res. 2013, 3 (4): 1574-1586.CrossRef
42.
Oh H, Kim DH, Cho JH, Kim YC: Hepatoprotective and free radical scavenging activities of phenolic petrosins and flavonoids isolated from Equisetum arvense. J Ethnopharmacol. 2004, 95 (2–3): 421-424.CrossRefPubMed
43.
Boyle SP, Dobson VL, Duthie SJ, Hinselwood DC, Kyle JA, Collins AR: Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study. Eur J Clin Nutr. 2000, 54 (10): 774-782. 10.1038/sj.ejcn.1601090.CrossRefPubMed
44.
Hubert DJ, Dawe A, Florence NT, Gilbert KD, Angele TN, Buonocore D, Finzi PV, Vidari G, Bonaventure NT, Marzatico F, Paul MF: In vitro hepatoprotective and antioxidant activities of crude extract and isolated compounds from Ficus gnaphalocarpa. Inflammopharmacology. 2011, 19 (1): 35-43. 10.1007/s10787-010-0070-4.CrossRefPubMed
45.
Domitrović R, Jakovac H, Vasiljev Marchesi V, Vladimir-Knežević S, Cvijanović O, Tadić Z, Romić Z, Rahelić D: Differential hepatoprotective mechanisms of rutin and quercetin in CCl(4)-intoxicated BALB/cN mice. Acta Pharmacol Sin. 2012, 33 (10): 1260-1270. 10.1038/aps.2012.62.CrossRefPubMedPubMedCentral
46.
Raja DP, Manickam VS, de Britto AJ, Gopalakrishnan S, Ushioda T, Satoh M, Tanimura A, Fuchino H, Tanaka N: Chemical and chemotaxonomical studies on Dicranopteris species. Chem Pharm Bull. 1995, 43: 1800-1803. 10.1248/cpb.43.1800.CrossRefPubMed
47.
Vilegas W, Sanommiya M, Rastrelli L, Pizza C: Isolation and structure elucidation of two new flavonoid glycosides from the infusion of Maytenus aquifolium leaves. Evaluation of the antiulcer activity of the infusion. J Agri Food Chem. 1999, 47: 403-406. 10.1021/jf980114i.CrossRef
48.
Yeşilada E, Takaishi Y: A saponin with anti-ulcerogenic effect from the flowers of Spartium junceum. Phytochemistry. 1999, 51: 903-908. 10.1016/S0031-9422(99)00198-3.CrossRefPubMed
49.
Yadav S, Bhadoria BK: Two dimeric flavonoids from Bauhinia purpurea. Indian J Chem. 2005, 44B: 2604-2607.
50.
Zakaria ZA, Abdul Hisam EE, Rofiee MS, Norhafizah M, Somchit MN, Teh LK, Salleh MZ: Antiulcer activity of the aqueous extract of Bauhinia purpurea leaf. J Ethnopharmacol. 2011, 137 (2): 1047-1054. 10.1016/j.jep.2011.07.038.CrossRefPubMed
Metadata
Title
Effect of methanol extract of Dicranopteris linearis against carbon tetrachloride- induced acute liver injury in rats
Authors
Farah Hidayah Kamisan
Farhana Yahya
Siti Syariah Mamat
Mohamad Fauzi Fahmi Kamarolzaman
Norhafizah Mohtarrudin
Teh Lay Kek
Mohd Zaki Salleh
Mohd Khairi Hussain
Zainul Amiruddin Zakaria
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2014
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-14-123

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