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BMC Pulmonary Medicine

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Open Access 01-12-2014 | Research article

T cell receptor-Vβ repertoires in lung and blood CD4⁺ and CD8⁺T cells of pulmonary sarcoidosis patients

Authors: Kerstin M Ahlgren, Tina Ruckdeschel, Anders Eklund, Jan Wahlström, Johan Grunewald

Published in: BMC Pulmonary Medicine | Issue 1/2014

Abstract

Background

Sarcoidosis patients have accumulations of activated CD4⁺ T cells in affected organs, such as the lungs. T cell receptor (TCR) Vβ-chain usage has been incompletely characterized in these patients.

Methods

We surveyed the TCR Vβ usage in CD4⁺ and CD8⁺ T cells in bronchoalveolar lavage (BAL) cells and peripheral blood mononuclear cells (PBMC) from 15 HLA-typed Scandinavian sarcoidosis patients. In addition, PBMC from 9 healthy volunteers and BAL cells from three of them were examined. Using 21 Vβ family-specific antibodies, we covered approximately 70% of all Vβ chains.

Results

In BAL T cells from sarcoidosis patients, we identified 16 CD4⁺ T cell expansions in 271 analyses (5.9%) and 21 CD8⁺ expansions in 240 analyses (8.7%). In PBMC we found 9 CD4⁺ expansions in 276 analyses (3.3%) and 12 CD8⁺ expansions out of 263 analyses (4.6%). Consistent with previous studies we found Vβ8 and Vβ16 expansions in sarcoidosis patients’ lungs. In addition, we found lung restricted Vβ22 expansions in three HLA DRB1 03⁺ patients. However, we found no statistically significant difference in frequency of expansions between patients and healthy controls.

Conclusions

The identified T cell expansions in present study indicate specific antigen recognition in the lungs of sarcoidosis patients.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2466/14/50/prepub

Title: T cell receptor-Vβ repertoires in lung and blood CD4+ and CD8+T cells of pulmonary sarcoidosis patients
Authors: Kerstin M Ahlgren
Tina Ruckdeschel
Anders Eklund
Jan Wahlström
Johan Grunewald
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Pulmonary Medicine / Issue 1/2014
Electronic ISSN: 1471-2466
DOI: https://doi.org/10.1186/1471-2466-14-50

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T cell receptor-Vβ repertoires in lung and blood CD4⁺ and CD8⁺T cells of pulmonary sarcoidosis patients

Abstract

Background

Methods

Results

Conclusions

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Abstract

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