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Open Access 01-12-2014 | Research article

Cisplatin sensitivity is enhanced in non-small cell lung cancer cells by regulating epithelial-mesenchymal transition through inhibition of eukaryotic translation initiation factor 5A2

Authors: Guodong Xu, Hui Yu, Xinbao Shi, Lebo Sun, Qingyun Zhou, Dawei Zheng, Huoshun Shi, Ni Li, Xianning Zhang, Guofeng Shao

Published in: BMC Pulmonary Medicine | Issue 1/2014

Abstract

Background

Epithelial-mesenchymal transition (EMT) has been believed to be related with chemotherapy resistance in non-small cell lung cancer (NSCLC). Recent studies have suggested eIF5A-2 may function as a proliferation-related oncogene in tumorigenic processes.

Methods

We used cell viability assays, western blotting, immunofluorescence, transwell-matrigel invasion assay, wound-healing assay combined with GC7 (a novel eIF5A-2 inhibitor) treatment or siRNA interference to investigate the role of eIF5A-2 playing in NSCLC chemotherapy.

Results

We found low concentrations of GC7 have little effect on NSCLC viability, but could enhance cisplatin cytotoxicity in NSCLC cells. GC7 also could reverse mesenchymal phenotype in NCI-H1299 and prevented A549 cells undergoing EMT after TGF-β1 inducement. eIF5A-2 knockdown resulted in EMT inhibition.

Conclusion

Our data indicated GC7 enhances cisplatin cytotoxicity and prevents the EMT in NSCLC cells by inhibiting eIF5A-2.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2466/14/174/prepub

Title: Cisplatin sensitivity is enhanced in non-small cell lung cancer cells by regulating epithelial-mesenchymal transition through inhibition of eukaryotic translation initiation factor 5A2
Authors: Guodong Xu
Hui Yu
Xinbao Shi
Lebo Sun
Qingyun Zhou
Dawei Zheng
Huoshun Shi
Ni Li
Xianning Zhang
Guofeng Shao
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Pulmonary Medicine / Issue 1/2014
Electronic ISSN: 1471-2466
DOI: https://doi.org/10.1186/1471-2466-14-174

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Cisplatin sensitivity is enhanced in non-small cell lung cancer cells by regulating epithelial-mesenchymal transition through inhibition of eukaryotic translation initiation factor 5A2

Abstract

Background

Methods

Results

Conclusion

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