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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2005

Open Access 01-12-2005 | Research article

A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium

Authors: Elien Moës, Leen Vijgen, Els Keyaerts, Kalina Zlateva, Sandra Li, Piet Maes, Krzysztof Pyrc, Ben Berkhout, Lia van der Hoek, Marc Van Ranst

Published in: BMC Infectious Diseases | Issue 1/2005

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Abstract

Background

Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.

Methods

Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.

Results

Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.

Conclusions

Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.
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Metadata
Title
A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
Authors
Elien Moës
Leen Vijgen
Els Keyaerts
Kalina Zlateva
Sandra Li
Piet Maes
Krzysztof Pyrc
Ben Berkhout
Lia van der Hoek
Marc Van Ranst
Publication date
01-12-2005
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2005
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-5-6

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