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Open Access 01-12-2014 | Research article

Genetic deletion in uncoupling protein 3 augments ¹⁸F-fluorodeoxyglucose cardiac uptake in the ischemic heart

Authors: Sara Gargiulo, Maria Piera Petretta, Adelaide Greco, Mariarosaria Panico, Michele Larobina, Matteo Gramanzini, Gabriele G Schiattarella, Giovanni Esposito, Mario Petretta, Arturo Brunetti, Alberto Cuocolo

Published in: BMC Cardiovascular Disorders | Issue 1/2014

Abstract

Background

We investigated the effects of uncoupling protein 3 (UCP3) genetic deletion on ¹⁸F-fluorodeoxyglucose (FDG) cardiac uptake by positron emission tomography (PET)/computed tomography (CT) dedicated animal system after permanent coronary artery ligation.

Methods

Cardiac ¹⁸F-FDG PET/CT was performed in UCP3 knockout (UCP3^-/-) and wild-type (WT) mice one week after induction of myocardial infarction or sham procedure.

Results

In sham-operated mice no difference in left ventricular (LV) volume was detectable between WT and UCP3^-/-. After myocardial infarction, LV volume was higher in both WT and UCP3^-/- compared to sham animals, with a significant interaction (p < 0.05) between genotype and myocardial infarction. In sham-operated animals no difference in FDG standardized uptake value (SUV) was detectable between WT (1.8 ± 0.6) and UCP3^-/- (1.8 ± 0.6). After myocardial infarction SUV was significantly higher in remote areas than in infarcted territories in both UCP3-/- and WT mice (both p < 0.01). Moreover, in remote areas, SUV was significantly higher (p < 0.001) in UCP3-/- as compared to WT, while in the infarcted territory SUV was comparable (p = 0.29). A significant relationship (r = 0.68, p < 0.001) between LV volume and SUV was found.

Conclusions

In a mice model of permanent coronary occlusion, UCP3 deficiency results in a metabolic shift that favored glycolytic metabolism and increased FDG uptake in remote areas.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2261/14/98/prepub

Title: Genetic deletion in uncoupling protein 3 augments 18F-fluorodeoxyglucose cardiac uptake in the ischemic heart
Authors: Sara Gargiulo
Maria Piera Petretta
Adelaide Greco
Mariarosaria Panico
Michele Larobina
Matteo Gramanzini
Gabriele G Schiattarella
Giovanni Esposito
Mario Petretta
Arturo Brunetti
Alberto Cuocolo
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Cardiovascular Disorders / Issue 1/2014
Electronic ISSN: 1471-2261
DOI: https://doi.org/10.1186/1471-2261-14-98

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