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BMC Immunology
Published in: BMC Immunology 1/2013

Open Access 01-12-2013 | Research article

Analysis of localized immune responses reveals presence of Th17 and Treg cells in cutaneous leishmaniasis due to Leishmania tropica

Authors: Gajendra Kumar Katara, Anand Raj, Rajesh Kumar, Kumar Avishek, Himanshu Kaushal, Nasim Akhtar Ansari, Ram Awatar Bumb, Poonam Salotra

Published in: BMC Immunology | Issue 1/2013

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Abstract

Purpose

The interaction between the Leishmania parasite and the host cell involves complex, multifaceted processes. The disease severity in cutaneous leishmaniasis (CL) is largely dependent on the causative species. Most of the information on immune responses in human CL is available with respect to L. major infection and is lacking for L. tropica species. In this study, we employed cytokine/chemokine/receptor membrane cDNA array to capture comprehensive picture of immuno-determinants in localized human tissue during L. tropica infection. Expression of selected molecules was evaluated by real time PCR in dermal lesion tissues at pre- and post treatment stages. Plasma IL-17 level was estimated by sandwich ELISA.

Results

The cDNA array analysis identified several immuno-determinants in tissue lesions of Indian CL including cytokines (IFN-γ, TNF-α, IL-1β, IL-10, IL-13), chemokines (IL-8, CCL2, CCL3, CCL4) and apoptotic molecules (Fas, TRAIL, IRF-1). Elevated mRNA levels of Th17 (IL-17, IL-23 and RORγt) and Treg (CD25, CTLA-4 and Foxp3) markers were observed in lesion tissues of CL patients compared to the control group, which subsided post treatment. Plasma IL-17 levels were found to be significantly higher in CL samples compared to controls.

Conclusions

In addition to defining comprehensive immunological responses inside lesion tissues of CL patients, our study demonstrated the presence of Th17 and Treg cells in CL caused by L. tropica.
Appendix
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Metadata
Title
Analysis of localized immune responses reveals presence of Th17 and Treg cells in cutaneous leishmaniasis due to Leishmania tropica
Authors
Gajendra Kumar Katara
Anand Raj
Rajesh Kumar
Kumar Avishek
Himanshu Kaushal
Nasim Akhtar Ansari
Ram Awatar Bumb
Poonam Salotra
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2013
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/1471-2172-14-52

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