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BMC Immunology
Published in: BMC Immunology 1/2013

Open Access 01-12-2013 | Research article

CAWS administration increases the expression of interferon γ and complement factors that lead to severe vasculitis in DBA/2 mice

Authors: Noriko Nagi-Miura, Daisuke Okuzaki, Kosuke Torigata, Minami A Sakurai, Akihiko Ito, Naohito Ohno, Hiroshi Nojima

Published in: BMC Immunology | Issue 1/2013

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Abstract

Background

Candida albicans water-soluble fraction (CAWS), a mannoprotein-β-glucan complex obtained from the culture supernatant of C. albicans NBRC1385, causes CAWS-mediated vasculitis (CAWS-vasculitis) in B6 and DBA/2 mice with mild and lethal symptoms, respectively. Why CAWS is lethal only in DBA/2 mice remains unknown.

Results

We performed DNA microarray analyses using mRNA obtained from peripheral blood mononuclear cells (PBMCs) of B6 and DBA/2 mice and compared their respective transcriptomes. We found that the mRNA levels of interferon-γ (Ifng) and several genes that regulate the complement system, such as C3, C4, Cfb, Cfh, and Fcna, were increased dramatically only in DBA/2 mice at 4 and 8 weeks after CAWS administration. The dramatic increase was confirmed by quantitative real-time polymerase chain reactions (qRT-PCR). Moreover, mRNA levels of immune-related genes, such as Irf1, Irf7, Irf9, Cebpb, Ccl4, Itgam, Icam1, and IL-12rb1, whose expression levels are known to be increased by Ifng, were also increased, but only in DBA/2 mice. By contrast, the mRNA level of Dectin-2, the critical receptor for the α-mannans of CAWS, was increased slightly and similarly in both B6 and DBA/2 mice after CAWS administration.

Conclusions

Taken together, our results suggest that CAWS administration induces Dectin-2 mediated CAWS-vasculitis in both B6 and DBA/2 mice and the expression of Ifng, but only in DBA/2 mice, which led to increased expression of C3, C4, Cfb, Cfh, and Fcna and an associated increase in lethality in these mice. This model may contribute to our understanding of the pathogenesis of severe human vasculitis.
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Metadata
Title
CAWS administration increases the expression of interferon γ and complement factors that lead to severe vasculitis in DBA/2 mice
Authors
Noriko Nagi-Miura
Daisuke Okuzaki
Kosuke Torigata
Minami A Sakurai
Akihiko Ito
Naohito Ohno
Hiroshi Nojima
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2013
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/1471-2172-14-44

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