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BMC Cardiovascular Disorders
Published in: BMC Cardiovascular Disorders 1/2020

Open Access 01-12-2020 | Abdominal Aortic Aneurysm | Research article

Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

Authors: Min Zhou, Zhenyu Shi, Liang Cai, Xu Li, Yong Ding, Tianchen Xie, Weiguo Fu

Published in: BMC Cardiovascular Disorders | Issue 1/2020

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Abstract

Background

This study aimed to identify the differentially expressed circular RNAs (circRNAs) between human abdominal aortic aneurysm (AAA) and the control group.

Methods

High-throughput sequencing was applied to determine the circRNA expression profiles of 4 paired aortic samples. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was carried out to testify 6 randomly selected dysregulated circRNAs. Kyoto Encyclopedia of Genes and Genomes and Gene ontology (GO) analysis were conducted for functional annotation of the parental genes. Additionally, interaction networks between circRNA and 5 putative microRNA (miRNA) partners were constructed.

Results

Finally, 411 differentially expressed circRNAs were discovered, including 266 downregulated and 145 upregulated circRNAs. Compared with the control group, the expression level of hsa (Homo sapiens) _circ_0005360 (LDLR) and hsa_circ_0002168 (TMEM189) were proved significantly lower in the AAA group by qRT-PCR. Regarding upregulated circRNAs, the most enriched GO molecular function, biological process and cellular component terms were poly(A) RNA binding, negative regulation of transcription from RNA polymerase II promoter and nucleoplasm, respectively. Moreover, circRNA/miRNA interaction networks showed that hsa_circ_0005360/miR-181b and hsa_circ_0002168/miR-15a axis might have a regulative role in human AAA.

Conclusions

This study revealed new circRNAs potentially related to the pathogenesis of AAA. Further experimental studies are warranted to clarify the potential molecular mechanisms.
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Metadata
Title
Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm
Authors
Min Zhou
Zhenyu Shi
Liang Cai
Xu Li
Yong Ding
Tianchen Xie
Weiguo Fu
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Cardiovascular Disorders / Issue 1/2020
Electronic ISSN: 1471-2261
DOI
https://doi.org/10.1186/s12872-020-01374-8

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