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International Journal of Diabetes in Developing Countries

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01-04-2020 | Insulins | Original Article

Association of adiponectin gene polymorphisms and their haplotypes with type 2 diabetes and related metabolic traits in an Iranian population

Authors: Hamid Reza Joshaghani, Parviz Kokhaei, Mehdi Barati, Abbas Pakdel, Ghorban Mohammadzadeh, Navid Bandegi, Ahmad Reza Bandegi

Published in: International Journal of Diabetes in Developing Countries | Issue 2/2020

Abstract

Introduction

Adiponectin is an adipocyte-secreted protein that contributes to glucose homeostasis. Contradictory reports are available on single nucleotide polymorphisms (SNPs) in the adiponectin gene and the risk of type 2 diabetes (T2D). We investigate the association of adiponectin gene SNPs (+45T/G and +276G/T) with serum adiponectin, insulin resistance, lipid profile, and T2D risk in an Iranian population.

Method

The +45T/G and +276G/T SNPs were genotyped in 211 non-familial T2D patients and 202 non-diabetic subjects by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan probe, respectively.

Results

T2D was associated with a decrease in serum adiponectin level. The G allele and the GG and TG genotypes of +45T/G SNP were more abundant than the T allele and the TT genotype in T2D patients compared with controls (p < 0.001). The risk of T2D in individuals with the GG and TG genotypes of +45T/G SNP was 4 and 2 times more than that with the TT genotype, respectively. There was no statistically significant difference in the frequencies of allele and genotype of +276G/T SNP between the control and T2D groups. The presence of +45G/+276G haplotype was associated with an increased risk of T2D (OR = 2.01, 95% CI = 1.34–3.03, p = 0.04).

Conclusion

Therefore, our results showed that +45T/G SNP is associated with the risk of T2D higher than +276G/T SNP in the studied population.

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Title: Association of adiponectin gene polymorphisms and their haplotypes with type 2 diabetes and related metabolic traits in an Iranian population
Authors: Hamid Reza Joshaghani
Parviz Kokhaei
Mehdi Barati
Abbas Pakdel
Ghorban Mohammadzadeh
Navid Bandegi
Ahmad Reza Bandegi
Publication date: 01-04-2020
Publisher: Springer India
Keywords: Insulins
Insulins
Type 2 Diabetes
Published in: International Journal of Diabetes in Developing Countries / Issue 2/2020
Print ISSN: 0973-3930
Electronic ISSN: 1998-3832
DOI: https://doi.org/10.1007/s13410-019-00785-4

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Abstract

Introduction

Method

Results

Conclusion

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