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International Journal of Diabetes in Developing Countries
Published in: International Journal of Diabetes in Developing Countries 3/2019

Open Access 01-07-2019 | Original Article

A non-targeted metabolomics study on different glucose tolerance states

Authors: Yan Gu, Peng Zang, Li-qin Li, Hui-zhi Zhang, Ji Li, Jin-xia Li, Yan-yan Yan, Shu-mao Sun, Jia Wang, Zhuang-yan Zhu

Published in: International Journal of Diabetes in Developing Countries | Issue 3/2019

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Abstract

A non-targeted metabolomics method was employed to study metabolic characteristics in subjects with different glucose tolerance. Plasma samples of 120 participants with normal glucose tolerance (NGT), impaired glucose regulation (IGR), and type 2 diabetes (T2D) were collected. Gas chromatography/mass spectrometry (GC/MS) was used to profile and compare the plasma metabolome among the three groups. Through the use of multivariate statistical analysis, we found distinct metabolome change from NGT to IGR and to T2D. ANOVA found that the IGR and T2D groups had perturbations of monosaccharide and lipid metabolism, disorders of glucogenic amino acids, and branched-chain amino acid catabolism. Furthermore, we also found that the levels of 2-hydroxybutyrate and 2-ketoisocaproate were progressively increased with glucose tolerance severity. The results from this study help us better understand the relationship between plasma metabolism and glucose tolerance states and also suggest that 2-hydroxybutyrate and 2-ketoisocaproate may be closely associated with the development of T2D.
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Metadata
Title
A non-targeted metabolomics study on different glucose tolerance states
Authors
Yan Gu
Peng Zang
Li-qin Li
Hui-zhi Zhang
Ji Li
Jin-xia Li
Yan-yan Yan
Shu-mao Sun
Jia Wang
Zhuang-yan Zhu
Publication date
01-07-2019
Publisher
Springer India
Published in
International Journal of Diabetes in Developing Countries / Issue 3/2019
Print ISSN: 0973-3930
Electronic ISSN: 1998-3832
DOI
https://doi.org/10.1007/s13410-018-0662-x

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