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01-04-2020 | Herpes Virus | Case Report

Next-generation sequencing technology as a powerful detection and semi-quantitative method for herpes simplex virus type 1 in pediatric encephalitis

Authors: Lin-lin Liu, Ling-yun Guo, Jie Dong, Wen-hua Huang, Li Zhao, Bing Liu, Xi-xi Zhang, Yong-qiang Jiang, Gang Liu

Published in: Journal of NeuroVirology | Issue 2/2020

Abstract

This case report presents a 1-year-old boy from China, with sudden onset of fever, convulsion, and sleepiness, screened for viral DNA in blood and cerebrospinal fluid (CSF) sample using next-generation sequencing (NGS) to diagnose herpes simplex virus type 1 (HSV-1) encephalitis, further validated by PCR. After acyclovir treatment, the patient’s symptom disappeared and HSV-1 DNA unique reads decreased from 4290 to zero in CSF, and from 23 to zero in blood detected by NGS. The clinical presentation and outcome were consistent with the pathogenic diagnostic results of NGS. NGS of CSF samples can be used as a diagnostic assay for HSV-1 encephalitis and also might be a semi-quantitative method for evaluation of treatment effect.

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Title: Next-generation sequencing technology as a powerful detection and semi-quantitative method for herpes simplex virus type 1 in pediatric encephalitis
Authors: Lin-lin Liu
Ling-yun Guo
Jie Dong
Wen-hua Huang
Li Zhao
Bing Liu
Xi-xi Zhang
Yong-qiang Jiang
Gang Liu
Publication date: 01-04-2020
Publisher: Springer International Publishing
Keywords: Herpes Virus
Encephalitis
Published in: Journal of NeuroVirology / Issue 2/2020
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-019-00803-5

At a glance: The STEP trials

Springer Medicine

Next-generation sequencing technology as a powerful detection and semi-quantitative method for herpes simplex virus type 1 in pediatric encephalitis

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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