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Tumor Biology
Published in: Tumor Biology 5/2016

01-05-2016 | Original Article

In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system

Authors: Fadwa Chaabane, Nadia Mustapha, Imen Mokdad-Bzeouich, Aicha Sassi, Soumaya Kilani-Jaziri, Marie-Geneviève Dijoux Franca, Serge Michalet, Mayssa Fathallah, Mounira Krifa, Kamel Ghedira, Leila Chekir-Ghedira

Published in: Tumor Biology | Issue 5/2016

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Abstract

The purpose of this study was to assess the antitumor and immunomodulatory effects of the aqueous extract from Daphne gnidium in mice-bearing melanoma tumor. Balb/C mice were subcutaneously implanted with B16-F10 cells and treated intraperitoneally with the aqueous extract at 200 mg/Kg b.w for 21 days. After euthanization on day 22, the tumors were weighed; lymphocyte proliferation, cytotoxic T lymphocyte (CTL), and natural killer (NK) cell activities were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring the lysosomal activity. In addition to its potential to inhibit the growth of the transplantable tumor, the aqueous extract remarkably induced splenocyte proliferation and both NK and CTL activities in tumor-bearing mice. The aqueous extract was also seen to have promoted lysosomal activity of host macrophages.
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Metadata
Title
In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system
Authors
Fadwa Chaabane
Nadia Mustapha
Imen Mokdad-Bzeouich
Aicha Sassi
Soumaya Kilani-Jaziri
Marie-Geneviève Dijoux Franca
Serge Michalet
Mayssa Fathallah
Mounira Krifa
Kamel Ghedira
Leila Chekir-Ghedira
Publication date
01-05-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 5/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-4492-x

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