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Insights into Imaging

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Open Access 01-08-2012 | Original Article

Stereology: a novel technique for rapid assessment of liver volume

Authors: Michael R. Torkzad, Agneta Norén, Joel Kullberg

Published in: Insights into Imaging | Issue 4/2012

Abstract

Background

The purpose of this study was to test the stereology method using several grid sizes for measuring liver volume and to find which grid provides an accurate estimate of liver volume.

Materials and methods

Liver volume was measured by volumetry in 41 sets of liver MRI. MRI was performed before and after different weight-reducing regimens. Grids of 3, 4, 5, and 6 cm were used to measure liver volume on different occasions by stereology. The liver volume and the changes in volume before and after treatment were compared between stereology and volumetry.

Results

There was no significant difference in measurements between stereology methods and volumetry (p > 0.05). The mean differences in liver volume between stereology based on 3-, 4-, 5-, and 6-cm grids and volumetry were 37, 3, 132, and 23 mL, respectively, and the differences in measurement of liver volume change were 21, 2, 19, and 76 mL, respectively. The mean time required for measurement by stereology was 59–190 s.

Conclusion

Stereology employing 3- and 4-cm grids can rapidly provide accurate results for measuring liver volume and changes in liver volume.

Main Messages

• Statistical methods can be used for measuring area/volume in radiology.

• Measuring liver volume by stereology by 4-cm grids can be done in less than two minutes.

• Follow-up of liver volume is highly accurate with stereological methods.

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Hori M, Suzuki K, Epstein ML, Baron RL (2011) Computed tomography liver volumetry using 3-dimensional image data in living donor liver transplantation: effects of the slice thickness on the volume calculation. Liver Transpl 17(12):1427–1436CrossRefPubMedPubMedCentral

Perkins JD (2009) Another formula to determine the prognosis of patients with acute liver failure. Liver Transpl 15(8):986–991CrossRefPubMed

Nakayama Y, Li Q, Katsuragawa S, Ikeda R, Hiai Y, Awai K, Kusunoki S, Yamashita Y, Okajima H, Inomata Y, Doi K (2006) Automated hepatic volumetry for living related liver transplantation at multisection CT. Radiology 240(3):743–748CrossRefPubMed

Karlo C, Reiner CS, Stolzmann P, Breitenstein S, Marincek B, Weishaupt D, Frauenfelder T (2010) CT- and MRI-based volumetry of resected liver specimen: comparison to intraoperative volume and weight measurements and calculation of conversion factors. Eur J Radiol 75(1):e107–e111CrossRefPubMed

Boyce RW, Dorph-Petersen KA, Lyck L, Gundersen HJ (2010) Design-based stereology: introduction to basic concepts and practical approaches for estimation of cell number. Toxicol Pathol 38(7):1011–1025CrossRefPubMed

Kamp S, Jemec GB, Kemp K, Kjeldsen CR, Stenderup K, Pakkenberg B, Dam TN (2009) Application of stereology to dermatological research. Exp Dermatol 18(12):1001–1009CrossRefPubMed

Mühlfeld C, Nyengaard JR, Mayhew TM (2010) A review of state-of-the-art stereology for better quantitative 3D morphology in cardiac research. Cardiovasc Pathol 19(2):65–82CrossRefPubMed

Dockery P, Fraher J (2007) The quantification of vascular beds: a stereological approach. Exp Mol Pathol 82(2):110–120CrossRefPubMed

Weibel ER, Hsia CC, Ochs M (2007) How much is there really? Why stereology is essential in lung morphometry. J Appl Physiol 102(1):459–467CrossRefPubMed

10.

Nyengaard JR, Gundersen HJ (2006) Direct and efficient stereological estimation of total cell quantities using electron microscopy. J Microsc 222(Pt 3):182–187CrossRefPubMed

11.

Torkzad M, Lindholm J, Martling A, Blomqvist L (2003) Retrospective measurement of different size parameters of non-radiated rectal cancer on MR images and pathology slides and their comparison. Eur Radiol 13(10):2271–2277CrossRefPubMed

12.

Kullberg J, Sundbom M, Haenni A, Freden S, Johansson L, Börnert P, Ahlström A, Ahlström H, Karlsson A (2011) Gastric bypass promotes more lipid mobilization than a similar weight loss induced by low-calorie diet. J Obes 2011:959–601CrossRef

13.

Aydinli B, Kantarci M, Polat KY, Unal B, Atamanalp SS, Durur I, Unal D, Akgun M (2006) Stereological evaluation of treatment response in patients with non-resectable hepatic alveolar echinococcosis using computed tomography via the Cavalieri method. Liver Int 26(10):1234–1240CrossRefPubMed

14.

Mazonakis M, Damilakis J, Mantatzis M, Prassopoulos P, Maris T, Varveris H, Gourtsoyiannis N (2004) Stereology versus planimetry to estimate the volume of malignant liver lesions on MR imaging. Magn Reson Imaging 22(7):1011–1016CrossRefPubMed

15.

Ghugre NR, Gonzalez-Gomez I, Shimada H, Coates TD, Wood JC (2010) Quantitative analysis and modelling of hepatic iron stores using stereology and spatial statistics. J Microsc 238(3):265–274CrossRefPubMed

16.

Yoshizumi T, Taketomi A, Kayashima H, Yonemura Y, Harada N, Ijichi H, Soejima Y, Nishizaki T, Maehara Y (2008) Estimation of standard liver volume for Japanese adults. Transplant Proc 40(5):1456–1460CrossRefPubMed

17.

Duran C, Aydinli B, Tokat Y, Yuzer Y, Kantarci M, Akgun M, Polat KY, Unal B, Killi R, Atamanalp SS (2007) Stereological evaluation of liver volume in living donor liver transplantation using MDCT via the Cavalieri method. Liver Transpl 13(5):693–698CrossRefPubMed

18.

Doherty CP, Fitzsimons M, Holohan T, Mohamed HB, Farrell M, Meredith GE, Staunton H (2000) Accuracy and validity of stereology as a quantitative method for assessment of human temporal lobe volumes acquired by magnetic resonance imaging. Magn Reson Imaging 18(8):1017–1025CrossRefPubMed

19.

Mazonakis M, Damilakis J, Maris T, Prassopoulos P, Gourtsoyiannis N (2002) Comparison of two volumetric techniques for estimating liver volume using magnetic resonance imaging. J Magn Reson Imaging 15(5):557–563CrossRefPubMed

20.

Reiner CS, Karlo C, Petrowsky H, Marincek B, Weishaupt D, Frauenfelder T (2009) Preoperative liver volumetry: how does the slice thickness influence the multidetector computed tomography- and magnetic resonance-liver volume measurements? J Comput Assist Tomogr 33(3):390–397CrossRefPubMed

Title: Stereology: a novel technique for rapid assessment of liver volume
Authors: Michael R. Torkzad
Agneta Norén
Joel Kullberg
Publication date: 01-08-2012
Publisher: Springer Berlin Heidelberg
Published in: Insights into Imaging / Issue 4/2012
Electronic ISSN: 1869-4101
DOI: https://doi.org/10.1007/s13244-012-0166-z

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Stereology: a novel technique for rapid assessment of liver volume

Abstract

Background

Materials and methods

Results

Conclusion

Main Messages

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Abstract

Background

Materials and methods

Results

Conclusion

Main Messages

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