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01-12-2019 | Original Article

Genotyping of Echinococcus granulosus isolates from livestock based on mitochondrial cox1 gene, in the Markazi province, Iran

Authors: Behnam Abedi, Amir Hossein Maghsood, Behzad Khansarinejad, Mohammad Fallah, Mohammad Matini, Shirzad Gholami, Abdol Sattar Pagheh, Reza Ghasemikhah

Published in: Journal of Parasitic Diseases | Issue 4/2019

Abstract

Hydatidosisis a parasitic disease caused by the larval stage of Echinococcus granulosus with different genotypes, and major complications in vital organs such as liver, lungs and, brain. Also, this parasite can infect animals and cause economic damages. Recently, some investigations indicated that the genetic variation of the parasite affects the antigenic, immunogenic and pathogenic features. Therefore, present study conducted to genotyping of the E. granulosus larva based on mitochondrial cox1 gene in livestock in the endemic areas of Markazi province, Iran. In this study, 49 hydatid cysts samples collected from 36 sheep, 11 goats and 2 cattle from different slaughterhouses of Markazi province in central part of Iran, 2017. The mitochondrial cox1 gene was amplified and genotyping were accomplished using sequence analysis. The sequencing analysis indicated that the main genotype G1 (61%) and G3 (37%) were identified. Also, one of the samples shows similarity with the G2 (2%) genotype. The results showed the statistically significant differences between the genotypes in different livestock (P < 0.05). This study indicated that the main genotypes of E. granulosus in Markazi province are G1 and G3 which are related to dog/sheep strain. Therefore, parasite control in dogs and sheep can reduce the risk of transmission of infection to humans.

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Title: Genotyping of Echinococcus granulosus isolates from livestock based on mitochondrial cox1 gene, in the Markazi province, Iran
Authors: Behnam Abedi
Amir Hossein Maghsood
Behzad Khansarinejad
Mohammad Fallah
Mohammad Matini
Shirzad Gholami
Abdol Sattar Pagheh
Reza Ghasemikhah
Publication date: 01-12-2019
Publisher: Springer India
Published in: Journal of Parasitic Diseases / Issue 4/2019
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-019-01132-4

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