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Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran

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Abstract

Nineteen hydatid cyst isolates collected from camels in central Iran were subjected to sequences analysis of mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes. A consensus sequence obtained containing 366 nucleotides for cox1 and 471 nucleotides for nad1 genes. Overall, the camel isolates indicated five different sequences in cox1 and nine in nad1 genes. The sequences analysis indicated that 26.3%, 42.1%, and 31.6% of isolates belonging to G1, G3, and G6 genotypes of Echinococcus granulosus, respectively. The isolates with G3 genotype indicated one cox1 sequence having 100% homology with reference G3 sequence (AN: M84663) and two different nad1 sequences, one having100% homology with reference G3 sequence (AN: AJ237634) and the other with a silent mutation (G to A) in position 279. The presence of G3 genotype (buffalo strain) of E. granulosus as dominant genotype in camels is emphasized. As G3 genotype has formerly been reported in human, the epidemiological role of camels is warranted in future surveys.

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Acknowledgments

The authors would like to thank Professor Robin Beat Gasser from the Department of Veterinary Science, University Of Melbourne, Australia for his kind advice and help for performing a portion of sequence samples in his laboratory. Also, thanks are due to Mr. Izadi and Dr. Ebrahimi in Najaf-Abad abattoir in Isfahan for their kind help in collecting samples. This study has been supported by the Tehran University of Medical Sciences Grant No. 85-04-27-4766. The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Science, Gorgan, Iran

    Mitra Sharbatkhori

  2. Department of Medical Parasitology and Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran

    Mitra Sharbatkhori, Hossein Mirhendi & Eshrat Beigom Kia

  3. Department of Medical Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

    Majid Fasihi Harandi & Elham Hajialilo

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  1. Mitra Sharbatkhori
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  5. Eshrat Beigom Kia
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Correspondence to Eshrat Beigom Kia.

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Sharbatkhori, M., Fasihi Harandi, M., Mirhendi, H. et al. Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran. Parasitol Res 108, 521–527 (2011). https://doi.org/10.1007/s00436-010-2092-7

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  • DOI: https://doi.org/10.1007/s00436-010-2092-7

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