Top

Published in:

01-06-2017 | Original Article

Larvicidal potency of marine actinobacteria isolated from mangrove environment against Aedes aegypti and Anopheles stephensi

Authors: S. Balakrishnan, P. Santhanam, M. Srinivasan

Published in: Journal of Parasitic Diseases | Issue 2/2017

Abstract

The marine soil samples were collected from different locations of Parangipettai mangrove ecosystem, Vellar estuary, southeast coast of India. Totally 30 different marine actinobacteria were isolated by serial dilution plate technique on starch casein agar medium. The isolated actinobacteria were investigated for their larvicidal activity against Aedes aegypti and Anopheles stephensi mosquitoes. Streptomyces fungicidicus, S. griseus, S. albus, S. alboflavus and S. rochei were identified as potential biocide producers. Based on the antimicrobial activity, five strains were chosen for larvicidal and pupicidal activity. Among the crude extracts tested, the S. alboflavus extract showed significant activity against Ae. aegypti (LC₅₀ 1.48 ± 0.09 and LC₉₀ 3.33 ± 0.22) and An. stephensi (LC₅₀ 1.30 ± 0.09 and LC₉₀ 3.13 ± 0.21). Five isolates have shown a most significant mortality rate of the Ae. aegypti and An. stephensi mosquito larvae. This is an ideal eco-friendly approach for the control of Japanese encephalitis vectors, Ae. aegypti and malarial vector An. stephensi.

Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–266CrossRef

Amer A, Mehlhorn H (2006a) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472CrossRefPubMed

Amer A, Mehlhorn H (2006b) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitol Res 99:478–490CrossRefPubMed

Ando K (1983) How to discover new antibiotics for insecticidal use. In: Takahashi T, Yoshioka H, Misato T, Matusunaka S (eds) Pesticide chemistry: human welfare and the environment. Natural products, Pergamon, New York, pp 253–259

Anonymous (1990) Biologically active KSB-1939L3 compound and its production-pesticide with insecticide and acaricide activity production by Streptomyces sp. culture. Biotechnol Abstr 9(19):58, Japan Patent, No. 273961, 1988

Antonio GE, Sánchez D, Williams T, Marina CF (2009) Paradoxical effects of sublethal exposure to the naturally derived insecticide spinosad in the dengue vector mosquito, Aedes aegypti. Pest Manag Sci 65(3):323–326CrossRefPubMed

Balakrishnan S, Indira K, Srinivasan M (2013) Mosquitocidal properties of Bacillus species isolated from mangroves of Vellar estuary, Southeast coast of India. J Parasit Dis. doi:10.1007/s12639-013-0371-9 PubMedPubMedCentral

Balakrishnan S, Srinivasan M, Mohanraj J (2014) Biosynthesis of silver nanoparticles from mangrove plant (Avicennia marina) extract and their potential mosquito larvicidal property. J Parasit Dis. doi:10.1007/s12639-014-0621-5 PubMed

Balaraman K (1995) Mosquito control potential of Bacillus thuringiensis subsp. israelensis and Bacillus sophaericus. ICMR Bull 25:45

Balaraman K, Rao USB, Rajagopalan PK (1979) Isolation of Metarrhizium anisopliae, Beauveria tenella and Fusarium oxysporum (Deuteromycetes) and their pathogenicity to Culex fatigans and Anopheles stephensi. Ind J Med Res 70:718

Balaraman K, Balasubramanian M, Manonmani LM (1983a) Bacillus thuringiensis H-14 (VCRC B-17) formulation as mosquito larvicide. Indian J Med Res 77:33PubMed

Balaraman K, Balasubramanian M, Jambulingam P (1983b) Field trial of Bacillus thuringiensis H-14 (VCRC B-17) against Culex and Anopheles larvae. Indian J Med Res 77:38PubMed

Berdy J (2005) Bioactive microbial metabolites—a personal view. J Antibiot 58:1–26CrossRefPubMed

Bream AS, Ghazal SA, El-Aziz ZKA, Ibrahim SY (2001) Insecticidal activity of selected actinomycetes strains against the Egyptian cotton leaf worm Spodoptera littoralis (Lepidoptera: Noctuidae). Mededelingen Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen Universiteit Gent 66(2a):503–544

Demain AL, Sanchez S (2009) Microbial drug discovery: 80 years of progress. J Antibiot 62:5–16CrossRefPubMed

Dhanasekaran D, Sakthi V, Thajuddin N, Panneerselvam A (2010) Preliminary evaluation of Anopheles mosquito larvicidal efficacy of mangrove actinobacteria. Int J Appl Biol Pharm Technol 1:374–381

Dhiman RC, Pahwa S, Dhillon GP, Dash AP (2010) Climate change and threat of vector-borne diseases in India: are we prepared? Parasitol Res 106(4):763–773CrossRefPubMed

El-Khawagh MA, Hamadah KhSh, El-Sheikh TM (2011) The insecticidal activity of actinomycete metabolites, against the mosquitoe Culex pipiens. Egypt Acad J Biol Sci 4(1):103–113

Finney DJ (1971) Probit analysis. Cambridge University Press, Cambridge, pp 76–80

Gadelhak GG, El-Tarabily KhA, Al-Kaabi FK (2005) Insect control using chitinolytic soil actinomycetes as biocontrol agents. Int J Agric Biol 7(4):627–633

Geetha I, Balaraman K (2001) Biomass and blastospore production in Beauveria bassiana (Bals) Vuill. as influenced by media components. J Biol Control 15(1):93

Geetha I, Prabakaran G, Paily KP, Manonmani AM, Balaraman K (2007) Characterisation of three mosquitocidal Bacillus strains isolated from mangrove forest. Biol Control 42:34–40CrossRef

Herbert AK (2010) The spinosyn family of insecticides: realizing the potential of natural products research. J Antibiot 63:101–111CrossRef

Hopwood DA, Buttner MJ, Kieser T, Charter KF (2000) Antibiotic production by Streptomyces. Pract Streptomyces Genet 1:1–42

Hussain AA, Mostafa SA, Ghazal SA, Ibrahim SY (2002) Studies on antifungal antibiotic and bioinsecticidal activities of some actinomycete isolates. Afr J Mycol Biotechnol 10:63–80

Jensen PR, Mincer TJ, Williams PG, Fenical W (2005) Marine actinomycete diversity and natural product discovery. Antonie Van Leeuwenhoek 87:43–48CrossRefPubMed

Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2008) Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171CrossRefPubMed

Karthik L, Kumar G, Bhaskara Rao KV (2010a) Comparison of methods and screening of biosurfactant producing marine actinobacteria isolated from Nicobar marine sediment. IIOAB J 1(2):221–227

Karthik L, Kumar G, Bhaskara Rao KV (2010b) Diversity of marine actinomycetes from Nicobar marine sediments and its antifungal activity. Int J Pharm Sci 2(1):199–203

Kenneth TM (1995) Part 111: Interaction 31. A comparative Study of the ability of fish to catch mosquito larvae. Rice-fish culture in china. International Development Research Centre. ISBN 1-55250-313-5

Leonard GC, Julius JM (2000) Review biopesticides: a review of their action, applications and efficacy. Pest Manag Sci 56:651–676CrossRef

Luz C, Tai MH, Santos AH, Rocha LF, Albernaz DA, Silva HH (2007) Ovicidal activity of entomopathogenic hyphomycetes on Aedes aegypti (Diptera: Culicidae) under laboratory conditions. J Med Entomol 44(5):799–804CrossRefPubMed

Manonmani AM, Balaraman K (2001) A high mosquitocidal Bacillus thuringienisis var. thompsoni. Curr Sci 80:779

Mehlhorn H, Schmahl G, Schmidt J (2005) Extract of the seeds of the plant Vitex agnus castus proven to be highly efficacious as a repellent against ticks, fleas, mosquitoes and biting flies. Parasitol Res 95(5):363–365CrossRefPubMed

Nadson GA (1903) Microorganisms as geological agents (Russian). From the Works of the investigation of the Slavian Mineral Waters, St. Petersburg

Nair MG, Putnam AR, Mishra SK, Mulks MH, Taft WH, Keller JE, Miller JR, Zhu PP, Meinhart JD, Lynn DG (1989) Faeriefungin: a new broad-spectrum antibiotic from Streptomyces griseus var. autotrophicus. J Nat Prod 52(4):797–809CrossRefPubMed

Pampiglione S, Majori G, Petrangeli G, Romi R (1985) Avermectins, MK-933 and MK-936, for mosquito control. Trans R Soc Trop Med Hyg 79:797–799CrossRefPubMed

Prabhakaran G, Padmanaban V, Balaraman K (2000) Comparative efficacy of UV screens in protecting the activity of a Bacillus thuringiensis formulation. J Biol Control 14:63

Pridham TG, Hesseltin CW, Benedict RG (1958) A guide for the classification of Streptomycetes according to selected groups. Placement of strains in morphological sections. App Microbiol 6:52

Rahuman AA, Bagavan A, Kamaraj C, Saravanan E, Zahir AA, Elango G (2009a) Efficacy of larvicidal botanical extracts against Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 104(6):1365–1372CrossRefPubMed

Rahuman AA, Bagavan A, Kamaraj C, Vadivelu M, Zahir AA, Elango G, Pandiyan G (2009b) Evaluation of indigenous plant extracts against larvae of Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 104(3):637–643CrossRefPubMed

Rajendran G, Sabesan S, Kuppusamy K, Balaraman K (1991) Evaluation of a Bacillus sphaericus formulation, Sphaeri fix for the control of Mansoniodes mosquitoes. Entomon 16:213

Rajesh K, Dhanasekaran D, Tyagi BK (2014) Mosquito survey and larvicidal activity of actinobacterial isolates against Culex larvae (Diptera: Culicidae). J Saudi Soc Agric Sci. doi:10.1016/j.jssas.2013.08.001

Ramanathan A, Shanmugam V, Rghuchander T, Samiyappan R (2002) Induction of systematic resistance in ragi against blast disease by Pseudomonas flourescens. Ann Plant Prot Sci 10:313–318

Rao KV, Chattopadhyay SK, Reddy GC (1990) Flavonoides with mosquito larval toxicity—tangeratin, daidzein and genistein crystal production, isolation and purification; Streptomyces spp. Culture. Insecticide. J Agric Food Chem 38:1427–1430CrossRef

Reddy PJ, Krishna D, Murthy US, Jamil K (1992) A microcomputer FORTRAN program for rapid determination of lethal concentration of biocides in mosquito control. CABIOS 8:209–213PubMed

Shi YF (2000) Advances of insecticidical microorganisms. Plant Prot 26:32–34

Shirling EB, Gottlieb D (1996) Methods for characterization of Streptomyces species. Int J Syst Evol Bacteriol 16:312–340

Sundarapandian S, Sundaram MD, Tholkappian P, Balasubramanian V (2002) Mosquitocidal properties of indigenous fungi and actinomycetes against Culex quinquefasciatus Say. J Biol Control 16:89–91

Vijayakumar R, Muthukumar C, Thajuddin N, Panneerselavam A (2007) Studies on the diversity of actinomycetes in the Palk Strait region of Bay of Bengal, India. Actinomycetologica 21:59–65CrossRef

Vijayakumar R, Murugesan S, Cholarajan A, Sakthi V (2010) Larvicidal potentiality of marine actinomycetes isolated from Muthupet Mangrove, Tamilnadu, India. Int J Microbiol Res 1(3):179–183

Vijayan V, Balaraman K (1991) Metabolites of fungi and actinomycetes active against mosquito larvae. Indian J Med Res 93:115–117PubMed

Weyland H (1969) Actinomycetes in North Sea and Atlantic Ocean sediments. Nature 23:858CrossRef

WHO (1996) Report of the WHO informal consultation on the evaluation on the testing of insecticides CTD/WHO PES/IC/96.1. p 69

Willoughby OH, Hoy M, Conley J (1987) Toxicity of pesticides to western predatory mite. Calif Agric 41:12–14

Xie MJ (1998) The perspective of the studies on microbial insecticides. J Liaoning Norm Univ Nat Sci 21:326–329

Zhang DF (1996) Recent developments in research and utilization of microorganisms. J Agric Sci 24(1):44–46

Zizka Z, Weiser J, Blumauerova M, Jizba J (1989) Ultra strucutural effects of macroterrolides of Streptomyces grieseus LKS-1 in tissues of Culex pipiens larvae-monactin, dinactin, triactin and nonactin preparation; insecticide activity. Cytobios 58:85–91PubMed

Zizka Z, Weiser J, Popov KB (1991) Effect of ichthyomycin on the ultra structure of tissues of Culex pipiens. Cytobios 65(260):31–37PubMed

Title: Larvicidal potency of marine actinobacteria isolated from mangrove environment against Aedes aegypti and Anopheles stephensi
Authors: S. Balakrishnan
P. Santhanam
M. Srinivasan
Publication date: 01-06-2017
Publisher: Springer India
Published in: Journal of Parasitic Diseases / Issue 2/2017
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-016-0812-3

ACC 2024 Congress Coverage

Heart Failure Video

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Larvicidal potency of marine actinobacteria isolated from mangrove environment against Aedes aegypti and Anopheles stephensi

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2017

Retro-bulbar cyst of Coenurus gaigeri in Barbari goats

Some probable factors affecting the malaria situation before and at the beginning of a pre-elimination program in southeastern Iran

A rare case of Theileria annulata induced corneal opacity in a calf

Isolated cysticercosis involving the anterior abdominal wall: a rare case report

Epidemiological studies on trichinellosis in pigs (Sus scofa) in India

Aphelenchus assamensis sp. nov. (Nematoda: Aphelenchida: Aphelenchoidea: Aphelenchidae) from Assam, North East India

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page