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01-04-2013 | Original Article

Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken

Authors: P. Venkateswara Rao, M. Raman, G. DhinakarRaj, S. Abdul Basith, S. Gomathinayagam

Published in: Journal of Parasitic Diseases | Issue 1/2013

Abstract

About 11 faecal samples from various regions of Tamil Nadu and Andhra Pradesh containing mixed spectrum of Eimeria species detected by morphometry viz. 15.4 × 11.2, 28.5 × 20.3, 31.1 × 18.5, 13.2 × 12.4, 20.8 × 17.5 and 22 × 18 μm for E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox and E. tenella respectively were taken for the study. The oocysts were concentrated and purified using faecal harvest protocol. The genomic DNA is extracted as per kit protocol. The amplicons of sizes 539 bp (E. tenella) and 460 bp (E. mitis) obtained could be visualised in a single lane for the multiplex PCR assay using sequence characterized amplified region primers.

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Title: Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken
Authors: P. Venkateswara Rao
M. Raman
G. DhinakarRaj
S. Abdul Basith
S. Gomathinayagam
Publication date: 01-04-2013
Publisher: Springer-Verlag
Published in: Journal of Parasitic Diseases / Issue 1/2013
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-012-0142-z

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Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken

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