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Indian Journal of Hematology and Blood Transfusion

Published in:

01-06-2016 | Correspondence

Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

Authors: Svetoslav Nanev Slavov, Katia Kaori Otaguiri, Dimas Tadeu Covas, Simone Kashima

Published in: Indian Journal of Hematology and Blood Transfusion | Special Issue 1/2016

Abstract

The infection of human parvovirus B19 (B19V) is a common event in the general population, including volunteer blood donors. In some cases it can be asymptomatic and can remain persistent for a long period of time. The objective of this study was to examine the B19V DNA prevalence and viral load in first-time volunteer blood donors. Blood samples were collected from 91 primary blood donors at the Regional Blood Center of Ribeirão Preto, Southeast Brazil. Viral detection and quantitation was performed by an in-house TaqMan^® real-time PCR with high sensitivity. B19V DNA was detected in one male blood donor (1.0 %) and was characterized by a very low viral load (537.36 copies/mL). Our studies demonstrate that B19V DNA at low titer may be present in apparently healthy individuals. Sensitive molecular diagnostic tools can be applied for the screening of fresh blood derived products in order to prevent transfusion-transmitted B19V infection.

Heegaard ED, Brown KE (2002) Human parvovirus B19. Clin Microbiol Rev 15:485–505CrossRefPubMedPubMedCentral

Slavov SN, Haddad SK, Silva-Pinto AC, Amarilla AA, Alfonso HL, Aquino VH, Covas DT (2012) Molecular and phylogenetic analyses of human parvovirus B19 isolated from Brazilian patients with sickle Cell disease, beta-thalassemia major and healthy blood donors. J Med Virol 84:1652–1665CrossRefPubMed

Juhl D, Steppat D, Görg S, Hennig H (2014) Parvovirus B19 infections and blood counts in blood donors. Transfus Med Hemother 41:52–59CrossRefPubMed

Corcioli F, Zakrzewska K, Rinieri A, Fanci R, Innocenti M, Civinini R, De Giorgi V, Di Lollo S, Azzi A (2008) Tissue persistance of p23-3arvovirus B19 genotypes in asymptomatic persons. J Med Virol 80:2005–2011CrossRefPubMed

Lefrère JJ, Servant-Delmas A, Candotti D, Mariotti M, Thomas I, Brossard Y, Lefrère F, Girot R, Allain JP, Laperche S (2005) Persistent B19 infection in immunocompetent individuals: implications for transfusion safety. Blood 106:2890–2895CrossRefPubMed

Matsukura H, Shibata S, Tani Y, Shibata H, Furuta RA (2008) Persistent infection by human parvovirus B19 in qualified blood donors. Transfusion 48:1036–1037CrossRefPubMed

Corcoran A, Doyle S (2004) Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19. J Med Microbiol 53:459–475CrossRefPubMed

Kleinman SH, Glynn SA, Lee TH, Tobler L, Montalvo L, Todd D, Kiss JE, Shyamala V, Busch MP, National Heart, Lung, Blood Institute, Retrovirus Epidemiology Donor Study (REDS-II) (2007) Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay. Transfusion 47:1756–1764CrossRefPubMed

Thomas I, Di Giambattista M, Gérard C, Mathys E, Hougardy V, Latour B, Branckaert T, Laub R (2003) Prevalence of human erythrovirus B19 DNA in healthy Belgian blood donors and correlation with specific antibodies against structural and non-structural viral proteins. Vox Sang 84:300–307CrossRefPubMed

10.

Ke L, He M, Li C, Liu Y, Gao L, Yao F, Li J, Bi X, Lv Y, Wang J, Hirsch ML, Li W (2011) The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers. Transfusion 51:1909–1918CrossRefPubMed

11.

Kooistra K, Mesman HJ, de Waal M, Koppelman MH, Zaaijer HL (2011) Epidemiology of high-level parvovirus B19V viraemia among Dutch blood donors, 2003–2009. Vox Sang 100(3):261–266CrossRefPubMed

12.

Juhl D, Görg S, Hennig H (2014) Persistence of Parvovirus B19 (B19V) DNA and humoral immune response in B19V-infected blood donors. Vox Sang 107:226–232CrossRefPubMed

13.

Lee TH, Kleinman SH, Wen L, Montalvo L, Todd DS, Wright DJ, Tobler LH, Busch MP, NHLBI, Retrovirus Epidemiology Donor Study-II (REDS-II) (2011) Distribution of parvovirus B19 DNA in blood compartments and persistence of virus in blood donors. Transfusion 51:1896–1908CrossRefPubMedPubMedCentral

14.

US Department of Health and Human Services, Food and Drug Administration, Center for Biologics Evaluation and Research. (2009) Nucleic Acid Testing (NAT) to Reduce the Possible Risk of Human Parvovirus B19 Transmission by Plasma-Derived Products. Available via: http://www.fda.gov/downloads/BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformation/Guidances/Blood/ucm078510.pdf

15.

Tsitsikas DA, Gallinella G, Patel S, Seligman H, Greaves P, Amos RJ (2014) Bone marrow necrosis and fat embolism syndrome in sickle cell disease: increased susceptibility of patients with non-SS genotypes and a possible association with human parvovirus B19 infection. Blood Rev 28:23–30CrossRefPubMed

Title: Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil
Authors: Svetoslav Nanev Slavov
Katia Kaori Otaguiri
Dimas Tadeu Covas
Simone Kashima
Publication date: 01-06-2016
Publisher: Springer India
Published in: Indian Journal of Hematology and Blood Transfusion / Issue Special Issue 1/2016
Print ISSN: 0971-4502
Electronic ISSN: 0974-0449
DOI: https://doi.org/10.1007/s12288-015-0607-1

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