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Journal of Cardiovascular Translational Research

Published in:

01-08-2015

Variability of Mouse Left Ventricular Function Assessment by 11.7 Tesla MRI

Authors: Laetitia Vanhoutte, Bernard Gallez, Olivier Feron, Jean-Luc Balligand, Hrag Esfahani, William d’Hoore, Stéphane Moniotte

Published in: Journal of Cardiovascular Translational Research | Issue 6/2015

Abstract

We studied intraobserver (n = 24), interobserver (n = 24) and interexperiment (n = 12) reproducibility of left ventricular (LV) mass and volume measurements in mice using an 11.7 T MRI system. The LV systolic function was assessed with a short-axis FLASH-cine sequence in 29 mice, including animals having undergone transverse aortic constriction. Bland-Altman and regression analysis were used to compare the different data sets. Reproducibility was excellent for the LV mass and end-diastolic volume (coefficient of variability (CoV) between 5.4 and 11.8 %), good for end-systolic volume (CoV 15.2–19.4 %) and moderate for stroke volume and ejection fraction (CoV 14.7–20.9 %). We found an excellent correlation between LV mass determined by MRI and ex vivo morphometric data (r = 0.92). In conclusion, LV systolic function can be assessed on an 11.7 T MRI scanner with high reproducibility for most parameters, as needed in longitudinal studies. However, data should be interpreted taking into account the moderate reproducibility of small volumes.

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Title: Variability of Mouse Left Ventricular Function Assessment by 11.7 Tesla MRI
Authors: Laetitia Vanhoutte
Bernard Gallez
Olivier Feron
Jean-Luc Balligand
Hrag Esfahani
William d’Hoore
Stéphane Moniotte
Publication date: 01-08-2015
Publisher: Springer US
Published in: Journal of Cardiovascular Translational Research / Issue 6/2015
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-015-9638-0

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