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Medical Oncology
Published in: Medical Oncology 6/2021

01-06-2021 | Breast Cancer | Original Paper

The function of Cav-1 in MDA-MB-231 breast cancer cell migration and invasion induced by ectopic ATP5B

Authors: Xinjie Dong, Yilei Li, Wei Li, Wenzhe Kang, Rong Tang, Wenyi Wu, Ziyi Xing, Lijuan Zhou

Published in: Medical Oncology | Issue 6/2021

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Abstract

Ectopic ATP5B, which is located in a unique type of lipid raft caveolar structure, can be upregulated by cholesterol loading. As the structural component of caveolae, Cav-1 is a molecular hub that is involved in transmembrane signaling. In a previous study, the ATP5B-specific binding peptide B04 was shown to inhibit the migration and invasion of prostate cancer cells, and the expression of ATP5B on the plasma membrane of MDA-MB-231 cells was confirmed. The present study investigated the effect of ectopic ATP5B on the migration and invasion of MDA-MB-231 cells and examined the involvement of Cav-1. Cholesterol loading increased the level of ectopic ATP5B and promoted cell migration and invasion. These effects were blocked by B04. Ectopic ATP5B was physically colocalized with Cav-1, as demonstrated by double immunofluorescence staining and coimmunoprecipitation. After Cav-1 knockdown, the migration and invasion abilities of MDA-MB-231 cells were significantly decreased, suggesting that Cav-1 influences the function of ectopic ATP5B. Furthermore, these effects were not reversed after treatment with cholesterol. We concluded that Cav-1 may participate in MDA-MB-231 cell migration and invasion induced by binding to ectopic ATP5B.
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Metadata
Title
The function of Cav-1 in MDA-MB-231 breast cancer cell migration and invasion induced by ectopic ATP5B
Authors
Xinjie Dong
Yilei Li
Wei Li
Wenzhe Kang
Rong Tang
Wenyi Wu
Ziyi Xing
Lijuan Zhou
Publication date
01-06-2021
Publisher
Springer US
Published in
Medical Oncology / Issue 6/2021
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-021-01519-5

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