Skip to main content
Top
Published in: Medical Oncology 10/2014

01-10-2014 | Original Paper

Validation of analytical breast cancer microarray analysis in medical laboratory

Authors: Amal Said Darweesh, Manal Louis Louka, Maha Hana, Shaymaa Rashad, Mohamed El-Shinawi, Ahmed Sharaf-Eldin, Samar Kamal Kassim

Published in: Medical Oncology | Issue 10/2014

Login to get access

Abstract

A previously reported microarray data analysis by RISS algorithm on breast cancer showed over-expression of the growth factor receptor (Grb7) and it also highlighted Tweety (TTYH1) gene to be under expressed in breast cancer for the first time. Our aim was to validate the results obtained from the microarray analysis with respect to these genes. Also, the relationship between their expression and the different prognostic indicators was addressed. RNA was extracted from the breast tissue of 30 patients with primary malignant breast cancer. Control samples from the same patients were harvested at a distance of ≥5 cm from the tumour. Semi-quantitative RT-PCR analysis was done on all samples. There was a significant difference between the malignant and control tissues as regards Grb7 expression. It was significantly related to the presence of lymph node metastasis, stage and histological grade of the malignant tumours. There was a significant inverse relation between expression of Grb7 and expression of both oestrogen and progesterone receptors. Grb7 was found to be significantly related to the biological classification of breast cancer. TTYH1 was not expressed in either the malignant or the control samples. The RISS by our group algorithm developed was laboratory validated for Grb7, but not for TTYH1. The newly developed software tool needs to be improved.
Literature
2.
go back to reference Li F, Brattain MG. Role of the survivin gene in pathophysiology. Am J Pathol. 2006;169:212–25. Li F, Brattain MG. Role of the survivin gene in pathophysiology. Am J Pathol. 2006;169:212–25.
3.
go back to reference Sharaf-Eldin A, Hana M, Kassim S, Rashad S. Association system for breast cancer microarray data. IJICIS. 2009;9:105–16. Sharaf-Eldin A, Hana M, Kassim S, Rashad S. Association system for breast cancer microarray data. IJICIS. 2009;9:105–16.
4.
go back to reference Cariou B, Bereziat V, Moncoq K, Kasus-Jacobi A, Perdereau D, Le Marcis V, Burnol AF. Regulation and functional roles of Grb14. Front Biosci. 2004;9:1626–36.PubMedCrossRef Cariou B, Bereziat V, Moncoq K, Kasus-Jacobi A, Perdereau D, Le Marcis V, Burnol AF. Regulation and functional roles of Grb14. Front Biosci. 2004;9:1626–36.PubMedCrossRef
5.
go back to reference Campbell HD, Kamei M, Claudianos C, Woollatt E, Sutherland GR, Suzuki Y, Hida M, Sugano S, Young IG. Human and mouse homologues of the Drosophila melanogaster tweety (tty) gene: a novel gene family encoding predicted transmembrane proteins. Genomics. 2000;68:89–92.PubMedCrossRef Campbell HD, Kamei M, Claudianos C, Woollatt E, Sutherland GR, Suzuki Y, Hida M, Sugano S, Young IG. Human and mouse homologues of the Drosophila melanogaster tweety (tty) gene: a novel gene family encoding predicted transmembrane proteins. Genomics. 2000;68:89–92.PubMedCrossRef
6.
go back to reference Toiyama Y, Mizoguchi A, Kimura K, Hiro J, Inoue Y, Tutumi T, Miki C, Kusunoki M. TTYH2: a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation. World J Gastroenterol. 2007;13:2717–21.PubMedCrossRefPubMedCentral Toiyama Y, Mizoguchi A, Kimura K, Hiro J, Inoue Y, Tutumi T, Miki C, Kusunoki M. TTYH2: a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation. World J Gastroenterol. 2007;13:2717–21.PubMedCrossRefPubMedCentral
7.
go back to reference AJCC. Breast. In: American Joint Committee on Cancer, Cancer Staging Manual. 6th ed; 2002; 171–180. AJCC. Breast. In: American Joint Committee on Cancer, Cancer Staging Manual. 6th ed; 2002; 171–180.
8.
go back to reference Carey LA, Perou CM, Livasy CA, Dressler LG, Cowan D, Conway K, Karaca G, Troester MA, Tse CK, Edmiston S, Deming SL, Geradts J, Cheang MC, Nielsen TO, Moorman PG, Earp HS, Millikan RC. Race, breast cancer subtypes, and survival in the Carolina breast cancer study. JAMA. 2006;295:2492–502.PubMedCrossRef Carey LA, Perou CM, Livasy CA, Dressler LG, Cowan D, Conway K, Karaca G, Troester MA, Tse CK, Edmiston S, Deming SL, Geradts J, Cheang MC, Nielsen TO, Moorman PG, Earp HS, Millikan RC. Race, breast cancer subtypes, and survival in the Carolina breast cancer study. JAMA. 2006;295:2492–502.PubMedCrossRef
9.
go back to reference Sen S, Sharma S, Gupta A, Gupta N, Singh H, Roychoudhury A, Mohanty S, Sen S, Nag TC, Tandon R. Molecular characterization of explant cultured human oral mucosal epithelial cells. Invest Ophthalmol Vis Sci. 2011;52:9548–54.PubMedCrossRef Sen S, Sharma S, Gupta A, Gupta N, Singh H, Roychoudhury A, Mohanty S, Sen S, Nag TC, Tandon R. Molecular characterization of explant cultured human oral mucosal epithelial cells. Invest Ophthalmol Vis Sci. 2011;52:9548–54.PubMedCrossRef
10.
go back to reference Lalkhen AG, McCluskey A. Clinical tests: sensitivity and specificity: continuing education in Anaesthesia. Crit Care Pain J. 2008;8:221–3. Lalkhen AG, McCluskey A. Clinical tests: sensitivity and specificity: continuing education in Anaesthesia. Crit Care Pain J. 2008;8:221–3.
11.
go back to reference Altieri DC. Validating survivin as a cancer therapeutic target: survivin, versatile modulation of cell division and apoptosis in cancer. Nat Rev Cancer. 2003;3:46–54.PubMedCrossRef Altieri DC. Validating survivin as a cancer therapeutic target: survivin, versatile modulation of cell division and apoptosis in cancer. Nat Rev Cancer. 2003;3:46–54.PubMedCrossRef
12.
go back to reference Bai T, Luoh SW. GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation. Carcinogenesis. 2008;29:473–9.PubMedCrossRef Bai T, Luoh SW. GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation. Carcinogenesis. 2008;29:473–9.PubMedCrossRef
13.
go back to reference Chu PY, Li TK, Ding ST, Lai IR, Shen TL. EGF-induced Grb7 recruits and promotes ras activity essential for the tumorigenicity of Sk-Br 3 breast cancer cells. J Biol Chem. 2010;285:29279–85.PubMedCrossRefPubMedCentral Chu PY, Li TK, Ding ST, Lai IR, Shen TL. EGF-induced Grb7 recruits and promotes ras activity essential for the tumorigenicity of Sk-Br 3 breast cancer cells. J Biol Chem. 2010;285:29279–85.PubMedCrossRefPubMedCentral
14.
go back to reference Giricz O, Calvo V, Pero SC, Krag DN, Sparano JA, Kenny PA. GRB7 is required for triple-negative breast cancer cell invasion and survival. Breast Cancer Res Treat. 2012;133:607–15.PubMedCrossRef Giricz O, Calvo V, Pero SC, Krag DN, Sparano JA, Kenny PA. GRB7 is required for triple-negative breast cancer cell invasion and survival. Breast Cancer Res Treat. 2012;133:607–15.PubMedCrossRef
15.
go back to reference Ramsey B, Bai T, Newell AH, Troxell M, Park B, Olson S, Keenan E, Shiuh-Wen L. Grb7 protein over-expression and clinical outcome in breast cancer. Breast Cancer Res Treat. 2011;127:659–69.PubMedCrossRef Ramsey B, Bai T, Newell AH, Troxell M, Park B, Olson S, Keenan E, Shiuh-Wen L. Grb7 protein over-expression and clinical outcome in breast cancer. Breast Cancer Res Treat. 2011;127:659–69.PubMedCrossRef
16.
go back to reference Vinatzer U, Dampier B, Streube B, Pacher M, Seewald MJ, Stratowa C, Kaserer K, Schreiber M. Expression of HER2 and the co-amplified genes Grb7 and MLN64 in human breast cancer: quantitative real-time reverse transcription- PCR as a diagnostic alternative to immunohistochemistry and fluorescence in situ hybridization. Clin Cancer Res. 2005;11:8348–57.PubMedCrossRef Vinatzer U, Dampier B, Streube B, Pacher M, Seewald MJ, Stratowa C, Kaserer K, Schreiber M. Expression of HER2 and the co-amplified genes Grb7 and MLN64 in human breast cancer: quantitative real-time reverse transcription- PCR as a diagnostic alternative to immunohistochemistry and fluorescence in situ hybridization. Clin Cancer Res. 2005;11:8348–57.PubMedCrossRef
17.
go back to reference Nadler Y, González AM, Camp RL, Rimm DL, Kluger HM, Kluger Y. Grb7 as a prognostic marker and therapeutic target in breast cancer. Ann Oncol. 2010;21:466–73.PubMedCrossRefPubMedCentral Nadler Y, González AM, Camp RL, Rimm DL, Kluger HM, Kluger Y. Grb7 as a prognostic marker and therapeutic target in breast cancer. Ann Oncol. 2010;21:466–73.PubMedCrossRefPubMedCentral
18.
go back to reference Parisi F, González AM, Nadler Y, Camp RL, Rimm DL, Kluger HM, Kluger Y. Benefits of biomarker selection and clinico-pathological covariate inclusion in breast cancer prognostic models. Breast Cancer Res. 2010;12:66–77.CrossRef Parisi F, González AM, Nadler Y, Camp RL, Rimm DL, Kluger HM, Kluger Y. Benefits of biomarker selection and clinico-pathological covariate inclusion in breast cancer prognostic models. Breast Cancer Res. 2010;12:66–77.CrossRef
19.
go back to reference Pradip D, Bouzyk M, Dey N, Leyland-Jones B. Dissecting GRB7-mediated signals for proliferation and migration in HER2 overexpressing breast tumor cells: GTP-ase rules. Am J Cancer Res. 2013;3:173–95.PubMedPubMedCentral Pradip D, Bouzyk M, Dey N, Leyland-Jones B. Dissecting GRB7-mediated signals for proliferation and migration in HER2 overexpressing breast tumor cells: GTP-ase rules. Am J Cancer Res. 2013;3:173–95.PubMedPubMedCentral
20.
go back to reference Sahlberg KK, Hongisto V, Edgren H, Mäkelä R, Hellström K, et al. The HER2 amplicon includes several genes required for the growth and survival of HER2 positive breast cancer cells. Mol Oncol. 2013;7:392–401.PubMedCrossRef Sahlberg KK, Hongisto V, Edgren H, Mäkelä R, Hellström K, et al. The HER2 amplicon includes several genes required for the growth and survival of HER2 positive breast cancer cells. Mol Oncol. 2013;7:392–401.PubMedCrossRef
Metadata
Title
Validation of analytical breast cancer microarray analysis in medical laboratory
Authors
Amal Said Darweesh
Manal Louis Louka
Maha Hana
Shaymaa Rashad
Mohamed El-Shinawi
Ahmed Sharaf-Eldin
Samar Kamal Kassim
Publication date
01-10-2014
Publisher
Springer US
Published in
Medical Oncology / Issue 10/2014
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-014-0201-7

Other articles of this Issue 10/2014

Medical Oncology 10/2014 Go to the issue