Top

Journal of Gastrointestinal Cancer

Published in:

01-12-2017 | Original Research

Anacyclus Pyrethrum Extract Exerts Anticancer Activities on the Human Colorectal Cancer Cell Line (HCT) by Targeting Apoptosis, Metastasis and Cell Cycle Arrest

Authors: Ali Mohammadi, Behzad Mansoori, Pooneh Chokhachi Baradaran, Sepideh Chokhachi Baradaran, Behzad Baradaran

Published in: Journal of Gastrointestinal Cancer | Issue 4/2017

Abstract

Introduction

Colorectal cancer is the second leading cause of cancer mortality in the USA. There are a number of medicinal plants triggering apoptosis response in cancer cells, thus have a therapeutic potential. On the other hand, due to traditional uses and availability of Anacyclus pyrethrum extract, we decided to evaluate the efficacy of this medicinal herb on human colorectal cancer cell line (HCT).

Materials and Methods

In the present study, the cytotoxic effects of Anacyclus pyrethrum extract were assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and trypan blue viability dye. Then, flow cytometry assay was exploited to measure cell death and apoptosis stage. The scratch test was exploited to assess the effect of Anacyclus pyrethrum on the migration of cancer cells. The expression levels of Caspase 3, Bcl-2, MMP1, and Vimentin genes were quantified by real-time PCR. Finally, cell cycle was analyzed by flow cytometry.

Results

MTT assay showed that Anacyclus pyrethrum extract significantly inhibited the cell growth. According to the flow cytometry assay result, the herbal extract was able to induce apoptosis in colorectal cancer cells. Our findings also demonstrated that the plant extract substantially increases the caspase 3 mRNA expression, while decreases Bcl-2, MMP1, and Vimentin. Cell cycle arrest occurred in G1 stage, due to the results of flow cytometry.

Conclusion

These results indicate that Anacyclus pyrethrum extract can successfully induce apoptosis in HCT cells. Therefore, it could be used as a novel therapeutic candidate for colorectal cancer treatment.

Gonzalez CA, Riboli E. Diet and cancer prevention: contributions from the European prospective investigation into cancer and nutrition (EPIC) study. Eur J Cancer. 2010;46(14):2555–62.CrossRefPubMed

Marmot M, Atinmo T, Byers T, Chen J, Hirohata T, Jackson A, et al. Food, nutrition, physical activity, and the prevention of cancer: a global perspective. Washington, DC: World Cancer Research Fund/American Institute for Cancer Research; 2007.

Willett WC, Stampfer MJ, Colditz GA, Rosner BA, Speizer FE. Relation of meat, fat, and fiber intake to the risk of colon cancer in a prospective study among women. N Engl J Med. 1990;323(24):1664–72.CrossRefPubMed

Wynder EL. The epidemiology of large bowel cancer. Cancer Res. 1975;35(11 Part 2):3388–94.PubMed

Reddy BS, Shamsudden A. Nutritional factors and colon cancer. Critical Reviews in Food Science & Nutrition. 1995;35(3):175–90.CrossRef

Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 1990;61(5):759–67.CrossRefPubMed

Mason JB. Nutritional chemoprevention of colon cancer. Semin Gastrointest Dis. 2002;13(3):143–53.

Negri E, D’avanzo B, Tavani A, Hill M, Giacosa A, Caygill C. The role of vegetables and fruit in cancer risk. Epidemiology of Diet and Cancer. 1994;327-34

Sunami E, Tsuno N, Osada T, Saito S, Kitayama J, Tomozawa S, et al. MMP-1 is a prognostic marker for hematogenous metastasis of colorectal cancer. Oncologist. 2000;5(2):108–14.CrossRefPubMed

10.

Zucker S, Vacirca J. Role of matrix metalloproteinases (MMPs) in colorectal cancer. Cancer Metastasis Rev. 2004;23(1–2):101–17.CrossRefPubMed

11.

Murray GI, Duncan ME, O’Neil P, Melvin WT, Fothergill JE. Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer. Nat Med. 1996;2(4):461–2.CrossRefPubMed

12.

Chan AT, Giovannucci EL. Primary prevention of colorectal cancer. Gastroenterology. 2010;138(6):2029–43. e10CrossRefPubMedPubMedCentral

13.

Mohammadi A, Mansoori B, Aghapour M, Baradaran B. Urtica dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells (PC3). Cellular and Molecular Biology (Noisy-le-Grand, France). 2015;62(3):78–83.

14.

Mohammadi, Ali, et al. The Urtica dioica extract enhances sensitivity of paclitaxel drug to MDA-MB-468 breast cancer cells. Biomed Pharmacother. 2016;83:835–842. doi:10.1016/j.biopha.2016.07.056.

15.

Cabrera C, Artacho R, Giménez R. Beneficial effects of green tea—a review. J Am Coll Nutr. 2006;25(2):79–99.CrossRefPubMed

16.

Annalakshmi R, Uma R, Chandran GS, Muneeswaran A. A treasure of medicinal herb-Anacyclus pyrethrum—a review. Indian J. Drugs Dis. 2012;1(3):59–67.

17.

Naderi NJ, Niakan M, Khodadadi E. Determination of antibacterial activity of Anacyclus pyrethrum extract against some of the oral bacteria: an in vitro study. Journal of Dentistry, Shiraz University of Medical Sciences. 2012;13(2):59–63.

18.

Sujith K, Darwin CR, Suba V. Memory-enhancing activity of Anacyclus pyrethrum in albino Wistar rats. Asian Pac J Trop Dis. 2012;2(4):307–11.

19.

Badhe S, Badhe R, Ghaisas M, Chopade V, Deshpande A. Evaluations of antidepressant activity of Anacyclus pyrethrum root extract. Int J Green Pharm. 2010;4(2):1–7.

20.

Sharma V, Boonen J, Spiegeleer BD, Dixit V. Androgenic and Spermatogenic Activity of Alkylamide‐Rich Ethanol Solution Extract of Anacyclus pyrethrum DC. Phytother Res. 2013;27(1):99–106.

21.

Ren W, Qiao Z, Wang H, Zhu L, Zhang L. Flavonoids: promising anticancer agents. Med Res Rev. 2003;23(4):519–34.

22.

Sylvester PW, Shah S. Antioxidants in dietary oils: their potential role in breast cancer prevention. Malay J Nutr. 2002;8:1–11.

23.

Baumeister P, Reiter M, Harréus U. Curcumin and other polyphenolic compounds in head and neck cancer chemoprevention. Oxid Med Cell Longev. 2012;2012:902716. doi:10.1155/2012/902716.

24.

Mohammadi A, Mansoori B, Goldar S, Shanehbandi D, Khaze V, Mohammadnejad L, et al. Effects of Urtica dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line (MDA-MB-468). Cellular and molecular biology (Noisy-le-Grand, France). 2015;62(2):62–7.

25.

Mohammadi A, Mansoori B, Aghapour M, Baradaran PC, Shajari N, Davudian S, et al. The Herbal Medicine Utrica Dioica Inhibits Proliferation of Colorectal Cancer Cell Line by Inducing Apoptosis and Arrest at the G2/M Phase. J Gastrointest Cancer. 2016;47(2):187–95.

26.

Amedei A, Munari F, Della Bella C, Niccolai E, Benagiano M, Bencini L, et al. Helicobacter pylori secreted peptidyl prolyl cis, trans-isomerase drives Th17 inflammation in gastric adenocarcinoma. Intern Emerg Med. 2014;9(3):303–9.

27.

Cory S, Adams JM. The Bcl2 family: regulators of the cellular life-or-death switch. Nat Rev Cancer. 2002;2(9):647–56.

28.

Su L, Zhou W, Park S, Wain JC, Lynch TJ, Liu G, et al. Matrix metalloproteinase-1 promoter polymorphism and lung cancer risk. Cancer Epidemiol Biomark Prev. 2005;14(3):567–70.

29.

John A, Tuszynski G. The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis. Pathol Oncol Res. 2001;7(1):14–23.

30.

Demant P. Cancer susceptibility in the mouse: genetics, biology and implications for human cancer. Nat Rev Genet. 2003;4(9):721–34.

31.

Yazdanparast R, Sadeghi H. Nucleic acid synthesis in cancerous cells under the effect of gnidilatimonoein from Daphne mucronata. Life Sci. 2004;74(15):1869–76.

32.

Fleisig H, Wong J. Measuring cell cycle progression kinetics with metabolic labeling and flow cytometry. J Vis Exp.: JoVE. 2012;(63):4045. doi:10.3791/4045.

33.

Roccio M, Schmitter D, Knobloch M, Okawa Y, Sage D, Lutolf MP, et al. Predicting stem cell fate changes by differential cell cycle progression patterns. Development. 2012;140(2):459–470.

34.

Volotskova O, Hawley TS, Stepp MA, Keidar M. Targeting the cancer cell cycle by cold atmospheric plasma. Sci Rep. 2012;2:636. doi:10.1038/srep00636.

35.

Owa T, Yoshino H, Okauchi T, Yoshimatsu K, Ozawa Y, Sugi NH, et al. Discovery of Novel Antitumor Sulfonamides Targeting G1 Phase of the Cell Cycle. J Med Chem. 1999;42(19):3789–3799

Title: Anacyclus Pyrethrum Extract Exerts Anticancer Activities on the Human Colorectal Cancer Cell Line (HCT) by Targeting Apoptosis, Metastasis and Cell Cycle Arrest
Authors: Ali Mohammadi
Behzad Mansoori
Pooneh Chokhachi Baradaran
Sepideh Chokhachi Baradaran
Behzad Baradaran
Publication date: 01-12-2017
Publisher: Springer US
Published in: Journal of Gastrointestinal Cancer / Issue 4/2017
Print ISSN: 1941-6628
Electronic ISSN: 1941-6636
DOI: https://doi.org/10.1007/s12029-016-9884-7

ACC 2024 Congress Coverage

Heart Failure Video

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Anacyclus Pyrethrum Extract Exerts Anticancer Activities on the Human Colorectal Cancer Cell Line (HCT) by Targeting Apoptosis, Metastasis and Cell Cycle Arrest

Abstract

Introduction

Materials and Methods

Results

Conclusion

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Introduction

Materials and Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 4/2017

Cystic Neuroendocrine Tumor of Retroperitoneum Origin—a Case Report

A Novel Approach: Local Resection for Ampullary GIST—Case Report and Review of Literature

Hepatocellular Carcinoma with Bone Metastases: Incidence, Prognostic Significance, and Management—Single-Center Experience

Primary Angiosarcoma of the Pancreas

A Road Less Traveled: Spinal Metastasis in Gastric Adenocarcinoma

Primary Hepatic Lymphoma: the Importance of Liver Biopsy

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page