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01-04-2018 | Original Article

Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial

Authors: Katherine V. Gates, Leigh G. Griffiths

Published in: Immunologic Research | Issue 2/2018

Abstract

Despite rabbits becoming an increasingly popular animal model, a flow cytometry panel that combines T cell markers (CD3, CD4, CD8, CD25, FOXP3) with a method for monitoring proliferation is lacking in this species. It has been shown that the rabbit model can be used to identify xenoantigens within bovine pericardium (BP), a common biological heart valve replacement material; however, these methods rely on monitoring the humoral immune response. The development of a rabbit T cell proliferation assay has utility in monitoring graft-specific cell-mediated immune responses toward bovine pericardium. Isolation and culture conditions were optimized to avoid cell death, red blood cell contamination, and non-specific proliferation. Effect of cell culture and stimulation on distribution and intensity of T cell markers was analyzed and compared between cells isolated from naïve and BP-immunized rabbits. Submaximal levels (0.25 μg/mL) of concavalin A were used to stimulate proliferation toward BP extract, with resultant proliferation compared between naïve and BP-immunized rabbits. Density stratification followed by ammonium potassium chloride (ACK) lysis yielded the greatest number of viable peripheral blood mononuclear cells with the least amount of erythrocyte contamination. Flat-bottomed plates were necessary to reduce non-specific proliferation in culture. T cells responded appropriately to maximal mitogenic stimulation (5 μg/mL concavalin A). Interestingly, immunization increased the intensity of FOXP3 in T regulatory cells compared to cells from naïve animals. With addition of submaximal levels of concavalin A, T cells from immunized rabbits proliferated in response to BP protein extract, while cells from naïve rabbits did not. In immunized rabbits, not only did more CD4⁺ T cells proliferate in response to BP re-stimulation, but the intensity of CD25 was increased indicating cell activation. This research provides a functional cell-mediated screening assay for assessment of BP-based biomaterials in rabbits, overcoming the limitations of previous humoral immune system-based assessments of biomaterial antigenicity in this important experimental animal species.

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Diemer P, Markoew S, Le DQ, Qvist N. Poly-epsilon-caprolactone mesh as a scaffold for in vivo tissue engineering in rabbit esophagus. Dis Esophagus. 2015;28(3):240–5. https://doi.org/10.1111/dote.12172.CrossRefPubMed

Mapara M, Thomas BS, Bhat KM. Rabbit as an animal model for experimental research. Dent Res J (Isfahan). 2012;9(1):111–8. https://doi.org/10.4103/1735-3327.92960.CrossRef

Cegielski M, Dziewiszek W, Zabel M, Dzięgiel P, Iżycki D, Zatoński M, et al. Experimental application of xenogenous antlerogenic cells in replacement of auricular cartilage in rabbits. Xenotransplantation. 2008;15(6):374–83. https://doi.org/10.1111/j.1399-3089.2008.00497.x.CrossRefPubMed

Griffiths LG, Choe LH, Reardon KF, Dow SW, Christopher Orton E. Immunoproteomic identification of bovine pericardium xenoantigens. Biomaterials. 2008;29(26):3514–20. https://doi.org/10.1016/j.biomaterials.2008.05.006.CrossRefPubMedPubMedCentral

Wong ML, Wong JL, Vapniarsky N, Griffiths LG. In vivo xenogeneic scaffold fate is determined by residual antigenicity and extracellular matrix preservation. Biomaterials. 2016;92:1–12. https://doi.org/10.1016/j.biomaterials.2016.03.024.CrossRefPubMedPubMedCentral

Dalgliesh AJ, Liu ZZ, Griffiths LG. Magnesium presence prevents removal of antigenic nuclear-associated proteins from bovine pericardium for heart valve engineering. Tissue Eng A. 2017;23(13–14):609–21. https://doi.org/10.1089/ten.tea.2016.0405.CrossRef

Xing JC, et al. Anti-infection tissue engineering construct treating osteomyelitis in rabbit tibia. Tissue Eng A. 2013;19(1–2):255–63. https://doi.org/10.1089/ten.tea.2012.0262.CrossRef

Cissell DD, Hu JC, Griffiths LG, Athanasiou KA. Antigen removal for the production of biomechanically functional, xenogeneic tissue grafts. J Biomech. 2014;47(9):1987–96. https://doi.org/10.1016/j.jbiomech.2013.10.041.CrossRefPubMed

Wong ML, Griffiths LG. Immunogenicity in xenogeneic scaffold generation: antigen removal vs. decellularization. Acta Biomater. 2014;10(5):1806–16. https://doi.org/10.1016/j.actbio.2014.01.028.CrossRefPubMedPubMedCentral

10.

Wong ML, Wong JL, Athanasiou KA, Griffiths LG. Stepwise solubilization-based antigen removal for xenogeneic scaffold generation in tissue engineering. Acta Biomater. 2013;9(5):6492–501. https://doi.org/10.1016/j.actbio.2012.12.034.CrossRefPubMed

11.

Gardin C, Ricci S, Ferroni L, Guazzo R, Sbricoli L, de Benedictis G, et al. Decellularization and Delipidation protocols of bovine bone and pericardium for bone grafting and guided bone regeneration procedures. PLoS One. 2015;10(7):e0132344. https://doi.org/10.1371/journal.pone.0132344.CrossRefPubMedPubMedCentral

12.

Hulsmann J, et al. Transplantation material bovine pericardium: biomechanical and immunogenic characteristics after decellularization vs. glutaraldehyde-fixing. Xenotransplantation. 2012;19(5):286–97. https://doi.org/10.1111/j.1399-3089.2012.00719.x.CrossRefPubMed

13.

Zhu D, Jin L, Wang X, Xu L, Liu T. Combined anticalcification treatment of bovine pericardium with decellularization and hyaluronic acid derivative. Biomed Mater Eng. 2014;24(1):741–9. https://doi.org/10.3233/BME-130862.PubMed

14.

Pagoulatou E, Triantaphyllidou IE, Vynios DH, Papachristou DJ, Koletsis E, Deligianni D, et al. Biomechanical and structural changes following the decellularization of bovine pericardial tissues for use as a tissue engineering scaffold. J Mater Sci Mater Med. 2012;23(6):1387–96. https://doi.org/10.1007/s10856-012-4620-8.CrossRefPubMed

15.

Stone KR, Walgenbach AW, Abrams JT, Nelson J, Gillett N, Galili U. Porcine and bovine cartilage transplants in cynomolgus monkey: I. A model for chronic xenograft rejection. Transplantation. 1997;63(5):640–5. https://doi.org/10.1097/00007890-199703150-00005.CrossRefPubMed

16.

Badylak SF, Gilbert TW. Immune response to biologic scaffold materials. Semin Immunol. 2008;20(2):109–16. https://doi.org/10.1016/j.smim.2007.11.003.CrossRefPubMedPubMedCentral

17.

Manji RA, Zhu LF, Nijjar NK, Rayner DC, Korbutt GS, Churchill TA, et al. Glutaraldehyde-fixed bioprosthetic heart valve conduits calcify and fail from xenograft rejection. Circulation. 2006;114(4):318–27. https://doi.org/10.1161/CIRCULATIONAHA.105.549311.CrossRefPubMed

18.

Simon P, Kasimir MT, Seebacher G, Weigel G, Ullrich R, Salzer-Muhar U, et al. Early failure of the tissue engineered porcine heart valve SYNERGRAFT in pediatric patients. Eur J Cardiothorac Surg. 2003;23(6):1002–6; discussion 1006. https://doi.org/10.1016/S1010-7940(03)00094-0.CrossRefPubMed

19.

Zaidi AH, et al. Preliminary experience with porcine intestinal submucosa (CorMatrix) for valve reconstruction in congenital heart disease: histologic evaluation of explanted valves. J Thorac Cardiovasc Surg. 2014;148(5):2216–4. 2225 e1CrossRefPubMed

20.

Courtman DW, Errett BF, Wilson GJ. The role of crosslinking in modification of the immune response elicited against xenogenic vascular acellular matrices. J Biomed Mater Res. 2001;55(4):576–86. https://doi.org/10.1002/1097-4636(20010615)55:4<576::AID-JBM1051>3.0.CO;2-9.CrossRefPubMed

21.

Dahm M, Lyman WD, Schwell AB, Factor SM, Frater RW. Immunogenicity of glutaraldehyde-tanned bovine pericardium. J Thorac Cardiovasc Surg. 1990;99(6):1082–90.PubMed

22.

Teixeira L, Marques RM, Águas AP, Ferreira PG. Regulatory T cells are decreased in acute RHDV lethal infection of adult rabbits. Vet Immunol Immunopathol. 2012;148(3–4):343–7. https://doi.org/10.1016/j.vetimm.2012.05.006.CrossRefPubMed

23.

Nesburn AB, Bettahi I, Dasgupta G, Chentoufi AA, Zhang X, You S, et al. Functional Foxp3+ CD4+ CD25(bright+) “natural” regulatory T cells are abundant in rabbit conjunctiva and suppress virus-specific CD4+ and CD8+ effector T cells during ocular herpes infection. J Virol. 2007;81(14):7647–61. https://doi.org/10.1128/JVI.00294-07.CrossRefPubMedPubMedCentral

24.

National Research Council. Guide for the Care and Use of Laboratory Animals: Eighth Edition. Washington, DC: The National Academies Press; 2011. p. 1–246.

25.

Wang Y, Bao J, Wu X, Wu Q, Li Y, Zhou Y, et al. Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization. Sci Rep. 2016;6(1):24779. https://doi.org/10.1038/srep24779.CrossRefPubMedPubMedCentral

26.

Dalgaard TS, Norup LR, Rubbenstroth D, Wattrang E, Juul-Madsen HR. Flow cytometric assessment of antigen-specific proliferation in peripheral chicken T cells by CFSE dilution. Vet Immunol Immunopathol. 2010;138(1–2):85–94. https://doi.org/10.1016/j.vetimm.2010.07.010.CrossRefPubMed

27.

Lin YZ, Deng XL, Shen N, Lü XL, Zhao LP, Kong XG, et al. Development of a CFSE-based flow cytometry for evaluating EIAV-stimulated proliferation of T lymphocytes. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008;24(11):1044–7.PubMed

28.

Andersen MN, et al. Elimination of Erroneous Results in Flow Cytometry Caused by Antibody Binding to Fc Receptors on Human Monocytes and Macrophages. Cytometry Part A. 2016;89a(11):1001–9.CrossRef

29.

Liu FC, Hoyt DB, Coimbra R, Junger WG. Proliferation assays with human, rabbit, rat, and mouse lymphocytes. In Vitro Cell Dev Biol Anim. 1996;32(9):520–3. https://doi.org/10.1007/BF02722976.CrossRefPubMed

30.

de Baetselier P, Vaeck M, de Smet W, Ron Y. Antigen-induced proliferation assay for rabbit T lymphocytes. I Characteristics of the response. Immunology. 1980;41(4):997–1003.PubMedPubMedCentral

31.

Ulmer AJ, Scholz W, Ernst M, Brandt E, Flad HD. Isolation and subfractionation of human peripheral blood mononuclear cells (PBMC) by density gradient centrifugation on Percoll. Immunobiology. 1984;166(3):238–50. https://doi.org/10.1016/S0171-2985(84)80042-X.CrossRefPubMed

32.

Mendez-David I, et al. A method for biomarker measurements in peripheral blood mononuclear cells isolated from anxious and depressed mice: beta-arrestin 1 protein levels in depression and treatment. Front Pharmacol. 2013;4:124.CrossRefPubMedPubMedCentral

33.

Maggioli MF, Palmer MV, Thacker TC, Vordermeier HM, Waters WR. Characterization of effector and memory T cell subsets in the immune response to bovine tuberculosis in cattle. PLoS One. 2015;10(4):e0122571. https://doi.org/10.1371/journal.pone.0122571.CrossRefPubMedPubMedCentral

34.

Janot C, Blancou J, Aubert MF. Cellular immunity in the red fox vaccinated against rabies. Studies with the lymphocyte transformation test. Comp Immunol Microbiol Infect Dis. 1982;5(1–3):129–37. https://doi.org/10.1016/0147-9571(82)90026-1.CrossRefPubMed

35.

Sloane ED, Muscoplat CC, Kaneene JM, Klausner DJ, Thoen CO, Johnson DW. In vitro stimulation of bovine peripheral blood lymphocytes: comparison of round- and flat-bottom microtiter plates for detection of tuberculin hypersensitivity. J Clin Microbiol. 1978;7(2):172–5.PubMedPubMedCentral

36.

Kim TJ, et al. Homotypic NK cell-to-cell communication controls cytokine responsiveness of innate immune NK cells. Sci Rep. 2014;4:7157.CrossRefPubMedPubMedCentral

37.

Uppal SS, Verma S, Dhot PS. Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking. Cytometry B Clin Cytom. 2003;52(1):32–6.CrossRefPubMed

38.

D'Acquisto F, Crompton T. CD3(+)CD4(−)CD8(−) (double negative) T cells: Saviours or villains of the immune response? Biochem Pharmacol. 2011;82(4):333–40. https://doi.org/10.1016/j.bcp.2011.05.019.CrossRefPubMed

39.

Rabinovitch PS, et al. Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry. J Immunol. 1986;137(3):952–61.PubMed

40.

Letourneau S, et al. IL-2-and CD25-dependent immunoregulatory mechanisms in the homeostasis of T-cell subsets. J Allergy Clin Immunol. 2009;123(4):758–62. https://doi.org/10.1016/j.jaci.2009.02.011.CrossRefPubMed

41.

van Herwijnen MJ, et al. In vivo induction of functionally suppressive induced regulatory T cells from CD4+CD25- T cells using an Hsp70 peptide. PLoS One. 2015;10(6):e0128373. https://doi.org/10.1371/journal.pone.0128373.CrossRefPubMedPubMedCentral

42.

Ivanova I, Seledtsova G, Mamaev S, Shishkov A, Seledtsov V. Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis. Hum Vaccin Immunother. 2014;10(5):1221–7. https://doi.org/10.4161/hv.28299.CrossRefPubMedPubMedCentral

43.

Sultani M, et al. Anti-inflammatory cytokines: important immunoregulatory factors contributing to chemotherapy-induced gastrointestinal mucositis. Chemother Res Pract. 2012;2012:490804.PubMedPubMedCentral

44.

Shalev I, Schmelzle M, Robson SC, Levy G. Making sense of regulatory T cell suppressive function. Semin Immunol. 2011;23(4):282–92. https://doi.org/10.1016/j.smim.2011.04.003.CrossRefPubMedPubMedCentral

45.

Itani HA, Harrison DG. Memories that last in hypertension. American Journal of Physiology-Renal Physiology. 2015;308(11):F1197–9. https://doi.org/10.1152/ajprenal.00633.2014.CrossRefPubMedPubMedCentral

46.

Croft M, Bradley LM, Swain SL. Naive versus memory CD4 T cell response to antigen. Memory cells are less dependent on accessory cell costimulation and can respond to many antigen-presenting cell types including resting B cells. J Immunol. 1994;152(6):2675–85.PubMed

47.

Croft M. Activation of naive, memory and effector T cells. Curr Opin Immunol. 1994;6(3):431–7. https://doi.org/10.1016/0952-7915(94)90123-6.CrossRefPubMed

48.

Wong ML, Leach JK, Athanasiou KA, Griffiths LG. The role of protein solubilization in antigen removal from xenogeneic tissue for heart valve tissue engineering. Biomaterials. 2011;32(32):8129–38. https://doi.org/10.1016/j.biomaterials.2011.07.030.CrossRefPubMed

49.

Christie D, Zhu J. Transcriptional regulatory networks for CD4 T cell differentiation. Curr Top Microbiol Immunol. 2014;381:125–72. https://doi.org/10.1007/82_2014_372.PubMedPubMedCentral

50.

Ciabattini A, Pettini E, Medaglini D. CD4(+) T cell priming as biomarker to study immune response to preventive vaccines. Front Immunol. 2013;4:421.CrossRefPubMedPubMedCentral

51.

Allan SE, Broady R, Gregori S, Himmel ME, Locke N, Roncarolo MG, et al. CD4+ T-regulatory cells: toward therapy for human diseases. Immunol Rev. 2008;223(1):391–421. https://doi.org/10.1111/j.1600-065X.2008.00634.x.CrossRefPubMed

52.

Driesen J, Popov A, Schultze JL. CD25 as an immune regulatory molecule expressed on myeloid dendritic cells. Immunobiology. 2008;213(9–10):849–58. https://doi.org/10.1016/j.imbio.2008.07.026.CrossRefPubMed

53.

Shipkova M, Wieland E. Surface markers of lymphocyte activation and markers of cell proliferation. Clin Chim Acta. 2012;413(17–18):1338–49. https://doi.org/10.1016/j.cca.2011.11.006.CrossRefPubMed

54.

Pinheiro A, Neves F, Lemos de Matos A, Abrantes J, van der Loo W, Mage R, et al. An overview of the lagomorph immune system and its genetic diversity. Immunogenetics. 2016;68(2):83–107. https://doi.org/10.1007/s00251-015-0868-8.CrossRefPubMed

Title: Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial
Authors: Katherine V. Gates
Leigh G. Griffiths
Publication date: 01-04-2018
Publisher: Springer US
Published in: Immunologic Research / Issue 2/2018
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-018-8985-8

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial

Abstract

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

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