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Published in:

01-07-2010

Transcriptional target-based expression cloning of immunoregulatory molecules

Authors: Rebecca L. Lamason, Stefanie M. Lew, Joel L. Pomerantz

Published in: Immunologic Research | Issue 1-3/2010

Abstract

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

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Title: Transcriptional target-based expression cloning of immunoregulatory molecules
Authors: Rebecca L. Lamason
Stefanie M. Lew
Joel L. Pomerantz
Publication date: 01-07-2010
Publisher: Humana Press Inc
Published in: Immunologic Research / Issue 1-3/2010
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-009-8148-z

At a glance: The STEP trials

Springer Medicine

Transcriptional target-based expression cloning of immunoregulatory molecules

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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